Anticancer effect of intracellular-delivered paclitaxel using novel pH-sensitive LMWSC-PCL di-block copolymer micelles

Publication date: Available online 14 November 2018Source: Journal of Industrial and Engineering ChemistryAuthor(s): Gyeong-Won Jeong, Woong-Gil Hong, Jun-Hyuk Anh, You-Jin Jeon, Suk Soon Choi, Tae-Il Son, Sang Chai Kim, Young-Il Jeong, Jae-Woon NahAbstractThe pH-sensitive LMWSC-PCL di-block copolymer (LMP) was synthesized by radical polymerization between LMWSC and PCL macromere using potassium persulfate (KPS), and paclitaxel (PTX)-encapsulated LMP (LMPPTX) nanoparticles were prepared by sonication and dialysis method. To investigate pH-sensitive effect of LMP, its particle size was measured in neutral-pH and acidic-pH condition. In addition, PTX from LMPPTX was released greater under acidic-pH. Additionally, the cytotoxicity of LMP and LMPPTX was evaluated by MTT assay and the cellular uptake was analyzed by fluorescence microscopy and flow cytometry. Overall, the results suggest that pH-sensitive LMPPTX nanoparticles are an excellent anticancer agent to inhibit tumor growth.
Source: Journal of Industrial and Engineering Chemistry - Category: Chemistry Source Type: research