TGF β mediates collagen production in human CRSsNP nasal mucosa‐derived fibroblasts through Smad2/3‐dependent pathway and CTGF induction and secretion

Transforming growth factor β1 (TGF‐β1) mediates collagen expression–production through a canonical Smad2/3‐dependent pathway and connective tissue growth factor (CTGF) induction and secretion in human nasal fibroblasts. Moreover, TGF‐β1, CTGF, and collagen are highly expressed in human chronic rhinosinusitis withou t nasal polyp (CRSsNP) nasal mucosa specimens, suggesting their roles in tissue remodeling during CRSsNP progression. AbstractChronic rhinosinusitis without nasal polyp (CRSsNP) is characterized by tissue remodeling and fibrosis. Transforming growth factor ‐β (TGF‐β) is considered a master switch in the induction of the profibrotic program which can induce fibroblasts to synthesize and contract extracellular matrix (ECM) proteins. A previous study has shown TGF‐β1 signaling and collagen overproduction in the CRSsNP, but the responsible cells and mechanism of action remain unclear. Therefore, this study was aimed to investigate the relationship between TGF‐β1 stimulation and collagen expression and to explore the role of connective tissue growth factor (CTGF) during the remodeling process using human CRSsNP nasal mucosa tissues and mu cosa‐derived fibroblasts as main materials. We found that TGF‐β1 and its isoforms could promote collagen protein expression. Concomitantly, TGF‐β1 caused CTGF expression and secretion. An addition of exogenous CTGF to fibroblasts also caused collagen expression. In accordance with these obse rvations, TGF...
Source: Journal of Cellular Physiology - Category: Cytology Authors: Tags: ORIGINAL RESEARCH ARTICLE Source Type: research