Tumor ‐suppressive microRNA‐10a inhibits cell proliferation and metastasis by targeting Tiam1 in esophageal squamous cell carcinoma

MicroRNA (miR) ‐10a inhibits esophageal squamous cell carcinoma (ESCC) progression by Tiam1; miR‐10a acts as tumor‐suppression by targeting Tiam1 in ESCC. AbstractAberrant microRNAs (miRNAs) expressions could contribute to the progression of numerous cancers, including esophageal squamous cell carcinoma, while miR ‐10a participates in multiple biological processes on cancers. However, the molecular mechanism of miR‐10a in esophageal squamous cell carcinoma (ESCC) has not been investigated. Herein, miR‐10a was significantly reduced in ESCC clinical tissues and ESCC cell lines (EC109 and TE‐3). In addit ion, immunohistochemistry indicated that the expressions of α‐SMA, Ki‐67, and PCNA in tumor tissues were higher than that of controls. In vitro, overexpression of miR‐10a dramatically suppressed cell proliferation and enhanced cell apoptosis, while the decrease of miR‐10a expressed the oppo site outcome. Specially, overexpression of miR‐10a caused a G0/G1 peak accumulation. Moreover, miR‐10a also negatively regulated ESCC cell migration and invasion. Furthermore, targetscan bioinformatics predictions and the dual‐luciferase assay confirmed that Tiam1 was a direct target gene of m iR‐10a. The statistical analysis showed Tiam1 was negatively in correlation with miR‐10a in ESCC patient samples. And silencing Tiam1 could lead to a decline on cell growth, invasion, and migration in ESCC cell lines, while it could enhance cell apoptosis and cause a G0/...
Source: Journal of Cellular Biochemistry - Category: Biochemistry Authors: Tags: RESEARCH ARTICLE Source Type: research