miR ‐483 inhibits bovine myoblast cell proliferation and differentiation via IGF1/PI3K/AKT signal pathway

MicroRNA ‐483 (miR‐483) inhibits bovine myoblast cell proliferation and differentiation by targeting insulin‐like growth factor 1 (IGF1) and downregulated the expression of key proteins in the PI3K/AKT signaling pathway. These results will provide an insight target in breeding strategies appealing to c attle industry. AbstractMicroRNAs (miRNAs) have been established to regulate skeletal muscle development in mammals. However, few studies have been conducted on the regulation of proliferation and differentiation of bovine myoblast cells by miRNAs. The aim of our study was to explore the function of miR ‐483 in cell proliferation and differentiation of bovine myoblast. Here, we found that miR‐483 declined in both proliferation and differentiation stages of bovine myoblast cells. During the proliferation phase, the overexpression of miR‐483 downregulated the cell cycle–associated genes cycli n‐dependent kinase 2 (CDK2), proliferating cell nuclear antigen (PCNA) messenger RNA (mRNA), and the protein levels. At the cellular level, cell cycle, cell counting kit ‐8, and 5‐ethynyl‐2´‐deoxyuridine results indicated that the overexpression of miR‐483 block cell proliferation. During differentiation, the overexpression of miR‐483 led to a decrease in the levels of the myogenic marker genesMyoD1 andMyoG mRNA and protein. Furthermore, the immunofluorescence analysis results showed that the number of MyHC ‐positive myotubes was reduced. In contrast, the oppos...
Source: Journal of Cellular Physiology - Category: Cytology Authors: Tags: ORIGINAL RESEARCH ARTICLE Source Type: research