Viruses, Vol. 10, Pages 625: Broad-Spectrum Antiviral Activity of an Ankyrin Repeat Protein on Viral Assembly against Chimeric NL4-3 Viruses Carrying Gag/PR Derived from Circulating Strains among Northern Thai Patients

Viruses, Vol. 10, Pages 625: Broad-Spectrum Antiviral Activity of an Ankyrin Repeat Protein on Viral Assembly against Chimeric NL4-3 Viruses Carrying Gag/PR Derived from Circulating Strains among Northern Thai Patients Viruses doi: 10.3390/v10110625 Authors: Supachai Sakkhachornphop Sudarat Hadpech Tanchanok Wisitponchai Chansunee Panto Doungnapa Kantamala Utaiwan Utaipat Jutarat Praparattanapan Wilai Kotarathitithum Sineenart Taejaroenkul Umpa Yasamut Koollawat Chupradit Sutpirat Moonmuang Vannajan Sanghiran Lee Khuanchai Suparatpinyo Chatchai Tayapiwatana Certain proteins have demonstrated proficient human immunodeficiency virus (HIV-1) life cycle disturbance. Recently, the ankyrin repeat protein targeting the HIV-1 capsid, AnkGAG1D4, showed a negative effect on the viral assembly of the HIV-1NL4-3 laboratory strain. To extend its potential for future clinical application, the activity of AnkGAG1D4 in the inhibition of other HIV-1 circulating strains was evaluated. Chimeric NL4-3 viruses carrying patient-derived Gag/PR-coding regions were generated from 131 antiretroviral drug-naïve HIV-1 infected individuals in northern Thailand during 2001–2012. SupT1, a stable T-cell line expressing AnkGAG1D4 and ankyrin non-binding control (AnkA32D3), were challenged with these chimeric viruses. The p24CA sequences were analysed and classified using the K-means clustering method. Among all the classes of virus classified usi...
Source: Viruses - Category: Virology Authors: Tags: Article Source Type: research