Characterization and CRISPR-based genotyping of clinical trh -positive Vibrio parahaemolyticus

ConclusionThetdh andtrh genes were not involved in urease production in thetrh+V. parahaemolyticus, and variation  of haemolytic activity detected inV. parahaemolyticus carrying only thetrh gene might be correlated to the sequence  variation withintrh1 andtrh2 genes. Additionally, biofilm production ofV. parahaemolyticus was not associated with harboring of virulence genes. For genotyping, CRISPR sequences combined with virulence genes can be used as genetic markers to differentiatetrh+V. parahaemolyticus strains.
Source: Gut Pathogens - Category: Microbiology Source Type: research

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Vibrio parahaemolyticus causes the most seafood attributed gastroenteritis outbreaks worldwide and studies on pathogenesis during passage through the human digestive fluids are limited. An in vitro continuous model system mimicking passage through saliva, gastric and intestinal fluid was used to study the survival, morphology and virulence-related gene expression of 60 pathogenic and non-pathogenic V. parahaemolyticus strains. The changes to these 3 characteristics for the 60 V. parahaemolyticus strains were minimal on passage through the saliva fluids. No V. parahaemolyticus strains survived passage through gastric fluid ...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
E. Blutt Sue E. Crawford Sasirekha Ramani David Y. Graham Robert L. Atmar Noroviruses, in the genus Norovirus, are a significant cause of viral gastroenteritis in humans and animals. For almost 50 years, the lack of a cultivation system for human noroviruses (HuNoVs) was a major barrier to understanding virus biology and the development of effective antiviral strategies. This review presents a historical perspective of the development of a cultivation system for HuNoVs in human intestinal epithelial cell cultures. Successful cultivation was based on the discovery of genetically-encoded host factors required for...
Source: Viruses - Category: Virology Authors: Tags: Review Source Type: research
The primary cause of parasitic gastroenteritis in small ruminants in temperate regions is the brown stomach worm, Teladorsagia circumcincta. Host immunity to this parasite is slow to develop, consistent with the ...
Source: Parasites and Vectors - Category: Microbiology Authors: Tags: Research Source Type: research
ConclusionsThis study showed that the whole-genome constellation of rotavirus G4P[6] strains from Korean neonates resembled a Wa-like genotype constellation. Additionally, rotavirus genotypes detected in Korean neonates had unique P[6] sequences, which may be the cause of Korean neonatal rotavirus infection.
Source: Gut Pathogens - Category: Microbiology Source Type: research
Noroviruses (NVs) are an important cause of acute gastroenteritis (AGE) worldwide. There are limited data on the prevalence and molecular characterization of NVs in children in Hohhot, China.
Source: BMC Infectious Diseases - Category: Infectious Diseases Authors: Tags: Research article Source Type: research
Condition:   Rotavirus Infection of Children Interventions:   Biological: TV P2-VP8;   Biological: Rotarix Sponsors:   PATH;   Bill and Melinda Gates Foundation;   SK Bioscience Not yet recruiting
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
Norovirus (NoV) is recognized as a leading cause of acute gastroenteritis (AGE) outbreaks in settings globally. Studies have shown that employees played an important role in the transmission mode during some N...
Source: BMC Infectious Diseases - Category: Infectious Diseases Authors: Tags: Research article Source Type: research
Abstract BACKGROUND: Absenteeism amongst pre-school children is often due to illnesses such as hand, foot, and mouth disease, acute gastroenteritis, cold and flu, which are easily spread amongst them. This is because of weak immunity and lack of knowledge on proper hand hygiene. This quasi-experimental study assessed the efficacy of an intervention consisting of a hand hygiene education programme, along with digital tools in bringing about a change in behaviour and health conditions amongst pre-school children in Klang Valley, Malaysia. METHODS: A total of 377 school children, male and female, aged 5-6 y...
Source: World Journal of Pediatrics : WJP - Category: Pediatrics Authors: Tags: World J Pediatr Source Type: research
ConclusionOur study showed high prevalence of enteric bacterial pathogens among food handlers. All isolates were susceptible to ciprofloxacin. However, a substential number of isolates were resistant to commonly prescribed antibiotics and the prevalence of MDR was high.
Source: Antimicrobial Resistance and Infection Control - Category: Infectious Diseases Source Type: research
Abstract SUMMARYCampylobacter is among the four main causes of gastroenteritis worldwide and has increased in both developed and developing countries over the last 10 years. The vast majority of reported Campylobacter infections are caused by Campylobacter jejuni and, to a lesser extent, C. coli; however, the increasing recognition of other emerging Campylobacter pathogens is urgently demanding a better understanding of how these underestimated species cause disease, transmit, and evolve. In parallel to the enhanced clinical awareness of campylobacteriosis due to improved diagnostic protocols, the applicati...
Source: Clinical Microbiology Reviews - Category: Microbiology Authors: Tags: Clin Microbiol Rev Source Type: research
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