Serenoa repens and N ‐acetyl glucosamine/milk proteins complex differentially affect the paracrine communication between endothelial and follicle dermal papilla cells

1)Paracrine communication between endothelial and follicle dermal papilla cells.
2)Characterization of the role of natural compounds on in vutro cocultures systems.
3)mechanistic background for the potential use of natural compounds as promoters of HF vascularization. AbstractCurrent treatments for hair follicle (HF) disruption are based on 5 ‐α reductase inhibitors and prostaglandin modulators. Botanicals and nutraceutical compounds interfere with hair loss or stimulate its partial regrowth. Here, we used in vitro cocultures to investigate the activity ofSerenoa repens (SR) andN‐acetyl glucosamine + milk proteins (NAG/Lac) on the paracrine interactions between human microvascular endothelial cells (HMVEC) and HF dermal papilla cells (FDPC). BothSR and NAG/Lac ‐induced endothelial tubulogenesis were enhanced by FDPC.SR promoted proliferation of both the cell types, while NAG/Lac was effective on endothelium. Vascular endothelial growth factor production, enhanced bySR, was further augmented by FDPC. In FDPC 5 ‐α reductase‐II and β‐catenin expressions were modified bySR and less by NAG/Lac, with no additional effect by HMVEC.SR and NAG/Lac prevented lipid peroxidation, whereas NAG/Lac was effective on interleukin 1 β production. Finally,SR and NAG/Lac differentially affected HMVEC permeability and tight junction proteins content. These data provide a mechanistic background for the potential use of these compounds as promoters of HF vascularization.

Source: Journal of Cellular Physiology - November 12, 2018 Category: Cytology Authors: Eleonora Bassino, Franco Gasparri, Luca Munaron Tags: ORIGINAL RESEARCH ARTICLE Source Type: research