MiR-876-5p regulates proliferation, migration and apoptosis of cervical cancer cells through targeting KPNA4

Publication date: Available online 11 November 2018Source: Pathology - Research and PracticeAuthor(s): Hongwei Lei, Jingbin Shi, Yun Teng, Chenghui Song, Lijuan Zou, Fuxiu Ye, Haichen ZhangAbstractMicroRNAs (miRNAs) play fundamental roles in the genesis and progression of various cancers. MiR-876-5p has demonstrated to be a tumor suppressor in several types of tumors. However, little is known about the expression pattern and biological roles of miR-876-5p in cervical cancer (CC). In present study, we assessed the expression level of miR-876-5p in cervical cancer tissues and normal tissues as well as in four cervical cancer cells and one normal cervical cells by qRT-PCR. MiR-876-5p expression was relatively lower in CC tissues and cells. To identify the function of miR-876-5p in regulating cervical cancer progression, gain-of-function assays were carried out. The cell proliferation and apoptosis were evaluated by MTT assay, colony formation assay and flow cytometry analysis. Cell migration and invasion was evaluated by transwell assay. Overexpression of miR-876-5p efficiently suppressed cell proliferation, migration and invasion but promoted cell apoptosis. Based on the bioinformatics analysis and mechanism experiments, KPNA4 was found to be a target of miR-876-5p. Rescue assays validated that KPNA4 mediated the tumor suppressive role of miR-876-5p in CC progression. Taken together, these results indicated that miR-876-5p acted as a tumor suppressor in the progression of cervi...
Source: Pathology Research and Practice - Category: Pathology Source Type: research