Invasive bronchial fibroblasts derived from asthmatic patients activate lung cancer A549 cells in vitro.

Invasive bronchial fibroblasts derived from asthmatic patients activate lung cancer A549 cells in vitro. Oncol Lett. 2018 Nov;16(5):6582-6588 Authors: Ryszawy D, Rolski F, Ryczek K, Catapano J, Wróbel T, Michalik M, Czyż J Abstract Epidemiological data suggests that there are functional links between bronchial asthma and lung carcinogenesis. Bronchial fibroblasts serve a prominent role in the asthmatic process; however, their involvement in lung cancer progression remains unaddressed. To estimate the effect of the asthmatic microenvironment on the invasiveness of lung cancer cells, the present study compared the behavior of human non-small cell lung cancer A549 cells exposed to the signals from human bronchial fibroblasts (HBFs) derived from non-asthmatic donors (NA HBFs) and from asthmatic patients (AS HBFs). NA HBFs did not significantly affect A549 motility, whereas AS HBFs and the media conditioned with AS HBF/A549 co-cultures increased Snail-1/connexin43 expression and motility of A549 cells. In contrast to NA HBFs, which formed A549-impenetrable lateral barriers, α-SMA+ AS HBFs actively infiltrated A549 monolayers and secreted chemotactic factors that arrested A549 cells within AS HBF/A549 contact zone. However, small sub-populations of A549 cells could release from this arrest and colonize distant regions of AS HBF monolayers. These data indicated that the interactions between lung cancer cells and HBFs in asthmatic bronchi...
Source: Oncology Letters - Category: Cancer & Oncology Tags: Oncol Lett Source Type: research