Application of a proton quantitative nuclear magnetic resonance spectroscopy method for the determination of actinodaphnine in Illigera aromatica and Illigera henryi

AbstractIlligera aromatica S. Z. Huang et S. L. Mo andIlligera henryi W. W. Sm., belonging to the genusIlligera (Hernandiaceae), are used as herbal medicines for promoting blood circulation and treating tuberculosis. Actinodaphnine, the major bioactive alkaloid, plays an important role in the quality controls of the herbs. In the present study, a rapid, simple, accurate, and precise proton quantitative nuclear magnetic resonance (1H-qNMR) method was developed to determine the content of actinodaphnine inI. aromatica andI. henryi. DMSO-d6 enabled satisfactory separation of the signals to be integrated in1H NMR spectrum. 1,4-Dinitrobenzene was selected as an internal standard. The limits of determination and quantitation were 0.005 and 0.038  mg/mL, respectively. This work implied that1H-qNMR represents a feasible alternative to HPLC-based methods for quantitation of actinodaphnine inI. aromatica andI. henryi and is suitable for the quality control ofI. aromatica andI. henryi.
Source: Journal of Natural Medicines - Category: Drugs & Pharmacology Source Type: research