Anthocyanin extract of Lannea microcarpa fruits stall oxidative rout associated with aflatoxin B1 hepatocarcinogenesis

Publication date: December 2013 Source:Food Bioscience, Volume 4 Author(s): T.O. Ajiboye , H.O. Raji , H.F. Muritala , O.B. Ojewuyi , M.T. Yakubu The capability of anthocyanins extract of Lannea microcarpa fruits to scavenge reactive oxygen species and stall aflatoxin B1-mediated oxidative rout on cellular proteins, lipids and DNA was investigated. Reactive oxygen species (ROS) scavenging potentials of the anthocyanins extract of Lannea microcarpa fruits (0.2–1.0mg/mL) was investigated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical, superoxide ion, hydrogen peroxide, hydroxyl radical and ferric ion reducing system. Anthocyanins extract at 1.0mg/mL scavenged DPPH, superoxide ion, hydrogen peroxide, and hydroxyl radical by 91%, 88%, 90% and 83%, respectively, it also reduced ferric ion significantly. The specific activities of reactive oxygen species detoxifying enzymes (superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase and glucose 6-phosphate dehydrogenase) were significantly (P<0.05) induced by Lannea microcarpa fruits anthocyanins. These inductions significantly (P<0.05) attenuated the aflatoxin B1-mediated decrease in reactive oxygen species detoxifying enzymes and compared favorably with Vitamin C. Aflatoxin B1-mediated elevation in the concentrations of conjugated dienes, lipid hydroperoxides, malondialdehyde, protein carbonyl and fragmented DNA were significantly (P<0.05) lowered by Lannea microcarpa fruits ...

Source: Food Bioscience - October 20, 2014 Category: Food Science Source Type: research