Isoform-specific interactions between meprin metalloproteases and the catalytic subunit of protein kinase A: Significance in acute and chronic kidney injury.

Isoform-specific interactions between meprin metalloproteases and the catalytic subunit of protein kinase A: Significance in acute and chronic kidney injury. Am J Physiol Renal Physiol. 2014 Oct 29;:ajprenal.00167.2014 Authors: Niyitegeka JM, Bastidas AC, Newman RH, Taylor SS, Ongeri EM Abstract Meprins metalloproteases are abundantly expressed in the brush border membranes of kidney proximal tubules. Meprins are implicated in ischemia/reperfusion (IR)-induced renal injury and diabetic nephropathy. The protein kinase A (PKA) signaling pathway modulates extracellular matrix metabolism in diabetic kidneys. The present study evaluated isoform-specific interactions between the catalytic subunit of PKA (PKA C) and meprins. To this end, cytosolic-enriched kidney proteins from meprin αβ double knockout mice, and purified forms of recombinant mouse PKA Cα, Cβ1, and Cβ2, were incubated with activated forms of either homomeric meprin A or meprin B. The cleaved protein products were subjected to SDS-PAGE and analyzed by coomassie staining and Western blot analysis. While meprin A only cleaved PKA Cβ1, meprin B cleaved all three PKA C isoforms. Analysis of the proteolytic fragments by mass spectrometry revealed that meprin A and B cleave the PKA C isoforms at defined sites, resulting in unique cleavage products. Michaelis-Menten enzyme kinetics demonstrated that meprin B-mediated cleavage of PKA Cα occurs at a rate consistent wi...
Source: Am J Physiol Renal P... - Category: Urology & Nephrology Authors: Tags: Am J Physiol Renal Physiol Source Type: research

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Authors: Morita K, Wang D, Baba R, Morimoto H, Song Y, Kanazawa T, Yoshida Y Abstract Although it is known that desert dust exacerbates allergic diseases, how it affects the onset of autoimmune diseases is unclear. No epidemiological investigations or animal experiments have been conducted so far to elucidate the effects of desert dust on autoimmune diseases. Here, we focused on particulate matter, such Asian sand dust (ASD) that has been known to cause adverse health effects in East Asian countries, and conducted animal experiments to examine how ASD influences type 1 diabetes (T1D), an autoimmune disease. An ASD ...
Source: Immunological Investigations - Category: Allergy & Immunology Tags: Immunol Invest Source Type: research
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Publication date: Available online 11 December 2019Source: International Journal of Nursing SciencesAuthor(s): Lei Cui, Weixia Chen, Xingxing Yu, Changping JuAbstractPurposePatients undergoing maintenance hemodialysis (MHD) have a higher prevalence of cognitive impairment and inferior cognitive performance than the general population, and those with cognitive impairment are at higher risk of death than those without cognitive impairment. Having diabetes has been associated with an increased risk of cognitive decline in end-stage kidney disease patients treated with peritoneal dialysis or kidney transplant. However, these f...
Source: International Journal of Nursing Sciences - Category: Nursing Source Type: research
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Source: Gut and Liver - Category: Gastroenterology Tags: Gut Liver Source Type: research
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Source: Neuroendocrinology Letters - Category: Endocrinology Tags: Neuro Endocrinol Lett Source Type: research
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