Isoform-specific interactions between meprin metalloproteases and the catalytic subunit of protein kinase A: Significance in acute and chronic kidney injury.

Isoform-specific interactions between meprin metalloproteases and the catalytic subunit of protein kinase A: Significance in acute and chronic kidney injury. Am J Physiol Renal Physiol. 2014 Oct 29;:ajprenal.00167.2014 Authors: Niyitegeka JM, Bastidas AC, Newman RH, Taylor SS, Ongeri EM Abstract Meprins metalloproteases are abundantly expressed in the brush border membranes of kidney proximal tubules. Meprins are implicated in ischemia/reperfusion (IR)-induced renal injury and diabetic nephropathy. The protein kinase A (PKA) signaling pathway modulates extracellular matrix metabolism in diabetic kidneys. The present study evaluated isoform-specific interactions between the catalytic subunit of PKA (PKA C) and meprins. To this end, cytosolic-enriched kidney proteins from meprin αβ double knockout mice, and purified forms of recombinant mouse PKA Cα, Cβ1, and Cβ2, were incubated with activated forms of either homomeric meprin A or meprin B. The cleaved protein products were subjected to SDS-PAGE and analyzed by coomassie staining and Western blot analysis. While meprin A only cleaved PKA Cβ1, meprin B cleaved all three PKA C isoforms. Analysis of the proteolytic fragments by mass spectrometry revealed that meprin A and B cleave the PKA C isoforms at defined sites, resulting in unique cleavage products. Michaelis-Menten enzyme kinetics demonstrated that meprin B-mediated cleavage of PKA Cα occurs at a rate consistent wi...
Source: Am J Physiol Renal P... - Category: Urology & Nephrology Authors: Tags: Am J Physiol Renal Physiol Source Type: research

Related Links:

Authors: Morita K, Wang D, Baba R, Morimoto H, Song Y, Kanazawa T, Yoshida Y Abstract Although it is known that desert dust exacerbates allergic diseases, how it affects the onset of autoimmune diseases is unclear. No epidemiological investigations or animal experiments have been conducted so far to elucidate the effects of desert dust on autoimmune diseases. Here, we focused on particulate matter, such Asian sand dust (ASD) that has been known to cause adverse health effects in East Asian countries, and conducted animal experiments to examine how ASD influences type 1 diabetes (T1D), an autoimmune disease. An ASD ...
Source: Immunological Investigations - Category: Allergy & Immunology Tags: Immunol Invest Source Type: research
Condition:   Bile Acid, Microbiota, Treatment of Diabetes Intervention:   Sponsor:   Chang Gung Memorial Hospital Not yet recruiting
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
Conditions:   Ketosis;   Type 2 Diabetes Interventions:   Dietary Supplement: Ketone monoester;   Dietary Supplement: Placebo Sponsor:   University of British Columbia Recruiting
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
Condition:   Type 2 Diabetes Mellitus Interventions:   Drug: Dapagliflozin Pill;   Drug: Empagliflozin Pill;   Drug: Placebo pill Sponsor:   Centro Universitario de Ciencias de la Salud, Mexico Not yet recruiting
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
Antioxidants&Redox Signaling,Volume 32, Issue 1, Page 60-77, January 1, 2020.
Source: Antioxidants and Redox Signaling - Category: Research Authors: Source Type: research
Publication date: Available online 11 December 2019Source: International Journal of Nursing SciencesAuthor(s): Lei Cui, Weixia Chen, Xingxing Yu, Changping JuAbstractPurposePatients undergoing maintenance hemodialysis (MHD) have a higher prevalence of cognitive impairment and inferior cognitive performance than the general population, and those with cognitive impairment are at higher risk of death than those without cognitive impairment. Having diabetes has been associated with an increased risk of cognitive decline in end-stage kidney disease patients treated with peritoneal dialysis or kidney transplant. However, these f...
Source: International Journal of Nursing Sciences - Category: Nursing Source Type: research
Conclusions: Compared with placebo, ghrelin agonists are effective and well-tolerated for the treatment of DG. PMID: 31816671 [PubMed - as supplied by publisher]
Source: Gut and Liver - Category: Gastroenterology Tags: Gut Liver Source Type: research
CONCLUSIONS: This study, in line with previous studies, shows that a diagnosis of T1DM is associated with a decreased risk of schizophrenia. This finding of an inverse association between T1DM and schizophrenia may bring an interesting piece, related to autoimmunity, into the schizophrenia-aetiology puzzle. PMID: 31816222 [PubMed - as supplied by publisher]
Source: Neuroendocrinology Letters - Category: Endocrinology Tags: Neuro Endocrinol Lett Source Type: research
Type 2 diabetes requires careful monitoring to reduce the risk of blood sugar spikes, which can lead to a host of health problems. Diet plays an essential role in regulating blood sugar and warding off serious threats and evidence backs regularly drinking a certain a certain tea to keep the condition in check.
Source: Daily Express - Health - Category: Consumer Health News Source Type: news
Pancreas-kidney transplant extends life expectancy for patients with type 1 diabetes and renal disease more than kidney transplantation alone, according to a study published inDiabetes Care.Healio
Source: Society for Endocrinology - Category: Endocrinology Source Type: news
More News: Diabetes | Endocrinology | Glomeruli | Men | Study | Urology & Nephrology