Dysregulated circular RNAs in medulloblastoma regulate proliferation and growth of tumor cells via host genes

The present study aims to investigate the expression profiles of circRNAs and related mechanisms in MB. We screened circRNAs expression profiles via HiseqTM Sequencer from four normal cerebellum and four MB samples. Subsequently, we validated expression levels of eight differential circRNAs by quantitative RT ‐PCR. Moreover, we silenced Circ‐SKA3 and Circ‐DTL with small interfere RNAs and over expressed their host genes to investigate their role in pathogenesis of medulloblastoma. The present study exploited circRNAs profiling in MB firstly and demonstrated that Circ‐SKA3 and Circ‐DTL played an important role in the tumorigenesis and development of MB and might be considered as novel and potential biomarkers for the diagnosis and new targets for intervention of MB AbstractCircular RNAs (circRNAs) have been demonstrated to be involved in various biological processes. Nevertheless, the function of circRNAs in medulloblastoma (MB) is still unknown. The present study aimed to investigate the expression profiles of circRNAs and related mechanisms for regulating the proliferation and growth of tumor cells in MB. The expression profiles of circRNAs were screened from four normal cerebellum and four MB samples using a HiSeq Sequencer. Bioinformatic analysis was employed to predict the interaction between circRNAs and mRNAs in MB. Subsequently, the expression levels of eight differential circRNAs [circ ‐SKA3 (hsa_circ_0029696), circ‐DTL (hsa_circ_0000179), circ‐CRTAM, ...
Source: Cancer Medicine - Category: Cancer & Oncology Authors: Tags: ORIGINAL RESEARCH Source Type: research