Azadirachta indica inhibits key enzyme linked to type 2 diabetes in vitro, abates oxidative hepatic injury and enhances muscle glucose uptake ex vivo

Publication date: January 2019Source: Biomedicine & Pharmacotherapy, Volume 109Author(s): Olakunle Sanni, Ochuko L. Erukainure, Chika I. Chukwuma, Neil A. Koorbanally, Collins U. Ibeji, Md. Shahidul IslamAbstractThe progression of secondary complications in type 2 diabetes (T2D) has been linked to oxidative stress caused by hyperglycemia. Therefore, the control of hyperglycemia is the main target in the treatment of diabetes. The present study investigated the scavenging and ameliorative potentials of different fractions of Azadirachta indica (A. indica) ethanol stem bark extract in Fe2+-induced oxidative injury in hepatic tissue as well as their ability to inhibit enzymes linked to diabetes and in enhancing muscle glucose uptake via some in vitro and ex vivo experimental models. The results revealed that the butanol fraction of the extract showed a significantly (p < 0.05) higher DPPH scavenging activity than the other fractions (IC50 0.0154 μg/mL), while the aqueous fraction showed the highest FRAP activity (IC50 25.32 μg/mL). Although all the fractions ameliorated Fe2+-induced oxidative injury in hepatic tissue by significantly reducing malondialdehyde (MDA) concentration in a dose dependent manner, the butanol fraction showed the highest activity in this regard. In addition, the activities of catalase and superoxide dismutase (SOD) were significantly improved by the butanol and dichloromethane fractions. Butanol and ethyl acetate fractions showed the highest...
Source: Biomedicine and Pharmacotherapy - Category: Drugs & Pharmacology Source Type: research