Ezetimibe inhibits dengue virus infection in Huh-7 cells by blocking the cholesterol transporter Niemann-Pick C1-like 1 receptor.

Ezetimibe inhibits dengue virus infection in Huh-7 cells by blocking the cholesterol transporter Niemann-Pick C1-like 1 receptor. Antiviral Res. 2018 Oct 31;: Authors: Osuna-Ramos JF, Reyes-Ruiz JM, Bautista-Carbajal P, Cervantes-Salazar M, Farfan-Morales CN, De Jesús-González LA, Hurtado-Monzon AM, Del Ángel RM Abstract Despite the importance of Dengue virus (DENV) infection in human health, there is not a fully effective vaccine or antiviral treatment against the infection. Since lipids such as cholesterol are required during DENV infection, its uptake and synthesis are increased in infected cells. Ezetimibe is an FDA-approved drug that reduces cholesterol uptake by inhibiting the endocytosis through Niemman-Pick C1-Like 1 (NPC1L1) receptor, expressed on the membrane of enterocytes and hepatocytes. Our results indicate that an increase in the amount of NPC1L1 occurs on the surface of Huh-7 cells during DENV infection, which correlates with an increase in cholesterol levels. Blockage of NPC1L1 with ezetimibe in concentrations up to 50 μM does not reduce cell viability but diminished total cellular cholesterol, the percentage of infected cells, viral yield, viral RNA and protein synthesis without affecting DENV binding and/or entry to Huh-7 cells. Moreover, ezetimibe inhibited DENV replicative complex formation and lipid droplets accumulation. All these results indicate that ezetimibe is an excellent drug to inhibit DENV inf...
Source: Antiviral Research - Category: Virology Authors: Tags: Antiviral Res Source Type: research

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CONCLUSION: the application is a technological tool with potential to improve the work process in vaccination rooms and to reach the goals of vaccine coverage. It synchronizes data with the National Immunization Program Information System, thus enabling the maintenance of people's vaccination history. PMID: 31826166 [PubMed - in process]
Source: Revista Latino-Americana de Enfermagem - Category: Nursing Authors: Tags: Rev Lat Am Enfermagem Source Type: research
Four physicians were among six people detained and released on Tuesday. US Customs and Border Patrol agents said that giving the vaccinations was'not feasible. 'Medscape Medical News
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This study aimed to examine the relationship of sociodemographic and anthropometric characteristics, and nutrient and food intakes with osteoarthritis prevalence in Korean elderly subjects with controlled dyslipidaemia. METHODS AND STUDY DESIGN: This study included 314 subjects aged ≥65 years who were diagnosed and treated for dyslipidaemia (data from the Seventh Korea National Health and Nutrition Examination Survey, 2016). Among them, 108 were also diagnosed with osteoarthritis. Sociodemographic, health, and nutritional data were analysed. RESULTS: Osteoarthritis prevalence was higher in females, highereduca...
Source: Asia Pacific Journal of Clinical Nutrition - Category: Nutrition Authors: Tags: Asia Pac J Clin Nutr Source Type: research
Publication date: Available online 11 December 2019Source: Trends in Endocrinology &MetabolismAuthor(s): Monica GomaraschiThe tumor microenvironment (TME) is an attractive target to develop novel strategies for hormone-dependent cancers. Several molecules in the TME can favor tumor development and progression, including lipoproteins. Lipoproteins are taken up by cancer cells, providing them with cholesterol and fatty acids. Cholesterol regulates cell signaling and it is converted into a series of bioactive metabolites, including hormones. The conflicting results of epidemiological and interventional studies suggest tha...
Source: Trends in Endocrinology and Metabolism - Category: Endocrinology Source Type: research
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(CNN) — Google users in the United States had a lot of questions about blood pressure, the keto diet and hiccups in 2019. Those topics were among the 10 most-searched health-related questions on the search engine this year, according to new data from Google. The list was based on search terms collected between January and early December. Last year, the top health-related questions Googled by people in the US included what is amyotrophic lateral sclerosis, or ALS, what is endometriosis and how long does weed stay in your urine. In 2017, what is lupus, how long does the flu last and what causes hiccups were some of the...
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Authors: Mühleck F, Laufs U Abstract Coronary artery disease (CAD) is the most frequent cause of morbidity and mortality worldwide. Lifestyle modifications and drug treatment of cardiovascular risk factors are able to effectively prevent CAD. The basis of prevention is the assessment of the individual cardiovascular risk, e.g. by using a validated risk score. Documented evidence for prevention of CAD is available for the control of hypertension using angiotensin-converting enzyme (ACE) inhibitors, angiotensin receptor blockers (ARB) and calcium antagonists, for the treatment of hypercholesterolemia using ...
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