Predomination shift of different P44-expressing Anaplasma phagocytophilum in infected HL-60, THP-1, NB4, and RF/6A cell lines.

In this study, we performed RNA-seq to investigate P44 expression of A. phagocytophilum propagated in 4 different cell lines. In infected HL-60 cells, P44-2b transcript was predominantly detected in first RNA-seq analysis (HL-60.1), but P44-23 transcript became predominated in second RNA-seq analysis at 1 month after additional passages (HL-60.2). We further analyzed P44 expression of A. phagocytophilum cultured in THP-1, NB4, and RF/6A cells through consecutively-repeated passages with own cell line for 1 year after transfer of A. phagocytophilum from infected HL-60 to respective cell lines. In those long-term cultures, P44-18, P44-78, and P44-51 became predominantly transcribed in infected THP-1, NB4, and RF/6A cells, respectively. Thus, the predomination shifts of different P44-expressing A. phagocytophilum might occur during cell cultures, probably due to host cell adaptation/selection/interaction, even in the same cell line at the different time points of sample harvest (HL-60.1 and HL-60.2).
PMID: 30381676 [PubMed - as supplied by publisher]

Source: Japanese Journal of Infectious Diseases - October 31, 2018 Category: Infectious Diseases Authors: Shimada M, Takamoto N, Su H, Sasahara H, Shimamura Y, Ando S, Ohashi N Tags: Jpn J Infect Dis Source Type: research