Aptazyme-mediated direct modulation of post-transcriptional sgRNA level for conditional genome editing and gene expression

In this study, a novel system was established to regulate the post-transcriptional sgRNA level by small molecule-controlled aptazyme. This system was shown to reduce the off-target effects caused by Cas9/sgRNA, while enabling precise temporal control over gene editing and regulatory activity. This new system could provide a potentially safer and more powerful tool for genome editing and therapeutic application.
Source: Journal of Biotechnology - Category: Biotechnology Source Type: research