Reciprocal Nerve Staining (RNS) for the concurrent detection of Choline Acetyltransferase and Myelin Basic Protein on paraffin-embedded sections

ConclusionsRoutine combination of choline acetyltransferase and myelin basic protein immunostaining provides a highly specific, highly contrasted paraffin-embedded sections where optical differentiation of myelinated motor fibers is easy and straightforward. This method will likely simplify and speed-up the routine histological study of nerve regeneration and will contribute a better identification of the nerve motor component.
Source: Journal of Neuroscience Methods - Category: Neuroscience Source Type: research