Crustin-capped selenium nanowires against microbial pathogens and Japanese encephalitis mosquito vectors – Insights on their toxicity and internalization

Publication date: January 2019Source: Journal of Trace Elements in Medicine and Biology, Volume 51Author(s): Ravichandran Rekha, Baskaralingam Vaseeharan, Sekar Vijayakumar, Muthukumar Abinaya, Marimuthu Govindarajan, Naiyf S. Alharbi, Shine Kadaikunnan, Jamal M. Khaled, Mohammed N. Al-anbrAbstractHerein, we reported a method to synthesize selenium nanowires (Cr-SeNWs) relying to purified cysteine-rich antimicrobial peptide crustin in presence of ascorbic acid. Cr-SeNWs were characterized by UV–vis, XRD, FTIR and Raman spectroscopy, as well as SEM, HR-TEM and EDAX. The UV–vis spectroscopy peak was noted at 350 nm. XRD showed the crystalline nature of Cr-SeNWs through diffraction peaks observed 2θ at 12° and 28° corresponding to (020), and (241) lattice planes, respectively. HR-TEM results shed light on the size of Cr-SeNWs, ranging from 17 to 47 nm. Raman spectroscopy and EDAX analysis of Cr-SeNWs showed presence of 57% selenium element. Furthermore, Cr-SeNWs showed higher antimicrobial activity on Gram-positive bacteria (Staphylococcus aureus, Enterococcus faecalis) over Gram-negative ones (Pseudomonas aeruginosa, Escherichia coli). The zone of inhibition was larger on S. aureus (50 μg/ml = 4.0 mm, 75 μg/ml = 7.2 mm) and E. faecalis (50 μg/ml = 3.1 mm, 75 μg/ml = 5.1 mm), over P. aeruginosa (50 μg/ml = 2.1 mm, 75 μg/ml = 4.8 mm), E. coli (50 μg/ml = 1.3 mm, 75 μg/ml = 4.3 mm) bacteria. The ant...
Source: Journal of Trace Elements in Medicine and Biology - Category: Biochemistry Source Type: research