Induction of Pancreatic Inflammation Accelerates Pancreatic Tumorigenesis in Mice.

Induction of Pancreatic Inflammation Accelerates Pancreatic Tumorigenesis in Mice. Methods Mol Biol. 2019;1882:287-297 Authors: Zhuang L, Zhan X, Bi Y, Ji B Abstract Pancreatitis is a major risk factor for the development of pancreatic cancer. In genetically engineered mouse models, induction of pancreatic inflammation dramatically accelerates oncogenic KRas-induced fibrosis, precancerous PanIN formation, and tumorigenesis. Here we describe simple methods of secretagogue-induced experimental acute and chronic pancreatitis, the most commonly used pancreatitis models, and their applications in pancreatic cancer research. Additionally, the preparation of primary pancreatic acinar cells is introduced. Primary acinar cells can be used to study the early events of pancreatic inflammation and pancreatic acinar-to-ductal (ADM) metaplasia. PMID: 30378063 [PubMed - in process]
Source: Mol Biol Cell - Category: Molecular Biology Authors: Tags: Methods Mol Biol Source Type: research