Identification of novel metabolomic biomarkers in an experimental model of septic acute kidney injury.

Identification of novel metabolomic biomarkers in an experimental model of septic acute kidney injury. Am J Physiol Renal Physiol. 2018 Oct 31;: Authors: Izquierdo-Garcia JL, Nin N, Cardinal-Fernandez P, Rojas Y, de Paula M, Granados R, Martínez-Caro L, Ruiz-Cabello J, Lorente JÁ Abstract The aim of this study is the identification of metabolomic biomarkers of sepsis and sepsis-induced acute kidney injury (AKI) in an experimental model. Pigs were anesthetized and monitored to measure mean arterial pressure (MAP), systemic blood flow (QT), mean pulmonary arterial pressure (MPAP), renal artery blood flow (QRA), renal cortical blood flow (QRC), and urine output (UO). Sepsis was induced at t=0 min by the administration of live Escherichia coli (n=6) or saline (n=8). At t=300 min, animals were sacrificed. Renal tissue, urine and serum samples were analyzed by Nuclear Magnetic Resonance (NMR) spectroscopy. Principal component analyses were performed on the processed NMR spectra to highlight kidney injury biomarkers. Sepsis was associated with decreased QT and MAP, and decreased QRA, QRC and urine output. Creatinine serum concentration and neutrophil gelatinase-associated lipocalin serum and urine concentrations increased. NMR-based metabolomics analysis found metabolic differences between control and septic animals: (i) in kidney tissue, increased lactate and nicotinuric acid, and decreased valine, aspartate, glucose and threonine; (ii) ...
Source: Am J Physiol Renal P... - Category: Urology & Nephrology Authors: Tags: Am J Physiol Renal Physiol Source Type: research