Effect of a novel antifungal peptide P852 on cell morphology and membrane permeability of Fusarium oxysporum

Publication date: Available online 31 October 2018Source: Biochimica et Biophysica Acta (BBA) - BiomembranesAuthor(s): Yuzhu Han, Jianjun Zhao, Bao Zhang, Qian Shen, Qingmao Shang, Pinglan LiAbstractP852, a novel cyclic peptide isolated from Bacillus amyloliquefaciens L-H15, showed potent antifungal activity against several major plant fungal pathogens including Fusarium oxysporum. To elucidate the antifungal mechanism, the impact of P852 on the cell morphology and membrane permeabilization of F. oxysporum was studied. By applying electron microscopy and fluorescent techniques, we showed that P852 treatment caused the morphological change of F. oxysporum cells and disrupted its cell structure, including formation of blebs, broken hyphae, deformation of membrane, intracellular organization disruption, pore formation, and cell lysis. Our findings provide insights into the mode of action of P852, which laying a foundation to develop P852 as a novel antifungal agent to control plant fungal pathogens.Graphical abstractA novel antifungal P852 induces membrane damage, leakage of cellular contents and hyphae fracture.

Source: Biochimica et Biophysica Acta (BBA) Biomembranes - November 1, 2018 Category: Biochemistry Source Type: research