Aflatoxin B1 metabolism: regulation by phase I and II metabolizing enzymes and chemoprotective agents

Publication date: Available online 29 October 2018Source: Mutation Research/Reviews in Mutation ResearchAuthor(s): Jiang Deng, Zhao Ling, Zhang Ni-Ya, Niel Alexander Karrow, Steven Krumm Christopher, Qi De-Sheng, Sun Lv-HuiAbstractAflatoxin B1 (AFB1) widely contaminates staple food and feed crops and is well-known as the most potent natural hepatocarcinogen in humans and domesticated animals. This review highlights significant advances in our understanding of the pivotal role of phase I and II metabolizing enzymes in the bioactivation and detoxification of AFB1 and its metabolites across species. In humans, cytochrome P450 (CYP) 1A2, CYP3 A4, CYP3 A5, and CYP3 A7 in liver and CYP2A13 in lung are essential for the bioactivation of AFB1 to the extremely toxic exo-AFB1-8,9-epoxide (AFBO), whereas CYP1A1, CYP1A2, CYP2A6, and CYP3 A4 are important in the turkey and duck, CYP1A1 and CYP2A6 are important in the chicken and quail, CYP3 A11 and CYP3 A13 are important in mice, and CYP2A5 are important in the hamster. In contrast, glutathione-S-transferase (GST) M1 and GSTT1 are primary responsible for detoxification of the AFB1 by catalyzing the conjugation of GSH to AFBO in humans, whereas GSTM2 in a nonhuman primate, GSTA3 in mice, GSTA5 in rats, and GSTA1, GSTA2, GSTA3 and GSTA4 in the turkey are important. Additionally, microsomal epoxide hydrolase (mEH) and aflatoxin-aldehyde reductase (AFAR) have also been shown to play key roles in AFB1 detoxification in the human, r...
Source: Mutation Research Reviews in Mutation Research - Category: Genetics & Stem Cells Source Type: research