Lifetime-based photoconversion of EGFP as a tool for FLIM

ConclusionsBesides potential FLIM artifacts caused by an unintended EGFP photoconversion, the controlled photoconversion turns EGFP to an excellent tool for kinetic FLIM applications. Since the photoconversion occurs in the lifetime domain, PC-EGFP can be easily distinguished from the unconverted tag by time-resolved detection while all other spectral channels stay free for multicolor labeling.GENERAL SIGNIFICANCE.The reported lifetime photoconversion lines up EGFP with other photoconvertible fluorescent proteins with special advantage for fluorescence lifetime imaging where lifetime-photoconvertible labels are scarce.Graphical abstract

Source: Biochimica et Biophysica Acta (BBA) General Subjects - October 29, 2018 Category: Biochemistry Source Type: research