Alpha-lipoic acid and coenzyme Q10 combination ameliorates experimental diabetic neuropathy by modulating oxidative stress and apoptosis

Publication date: Available online 28 October 2018Source: Life SciencesAuthor(s): Nasrin Sadeghiyan Galeshkalami, Mohammad Abdollahi, Rezvan Najafi, Maryam Baeeri, Akram Jamshidzade, Reza Falak, Mohammad Davoodzadeh Gholami, Gholamreza Hassanzadeh, Tahmineh Mokhtari, Shokoufeh Hassani, Mahban Rahimifard, Asieh HosseiniAbstractAimsDiabetic neuropathy (DN) is the most common complication of diabetes. Neuroprotective effects of alpha lipoic acid (ALA) and coenzyme Q10 (CoQ10) has been previously shown in DN, but underlying mechanisms involved not been exactly found. The present study explored the neuroprotective effects of ALA and Q10 combination in experimental DN by ameliorating oxidative stress and apoptosis.Main methodsWe investigated the effects of CoQ10 (10 mg/kg, orally, five weeks) and/or ALA (100 mg/kg, orally, five weeks) in STZ (45 mg/kg, i.p.)- induced DN in rats. After treatments motor function, oxidative stress biomarkers, ATP levels, expression of caspase 3 and UCP2 proteins were assessed by open-field, biochemical and ELISA methods and Western blot analysis. Dorsal root ganglion (DRG) neurons were histologically examined using H&E staining method.Key findingsALA and/or CoQ10 treatment significantly (p < 0.05) attenuated DN – induced motor function deficiency by modulating distance moved and velocity. ALA and/or CoQ10 treatment dramatically suppressed DN – induced oxidative stress which was associated with decrease in LPO and ROS and increase in ...
Source: Life Sciences - Category: Biology Source Type: research