Chromatin Immunoprecipitation in Skeletal Myoblasts.

Chromatin Immunoprecipitation in Skeletal Myoblasts.
Methods Mol Biol. 2019;1889:43-54
Authors: Rao VK, Shankar SR, Taneja R
Abstract
Chromatin immunoprecipitation (ChIP) is a powerful and sensitive technique that is widely used to study DNA-protein interactions. It enables an unbiased genome-wide analysis of transcriptional changes during several biological processes including cellular differentiation. Here, we describe a step-by-step protocol to identify histone modifications, transcription factor, and co-factor binding to chromatin in skeletal myoblasts. We discuss critical steps during cell harvesting, sonication, and immunoprecipitation and provide notes to evade common pitfalls.
PMID: 30367408 [PubMed - in process]

Source: Mol Biol Cell - October 28, 2018 Category: Molecular Biology Authors: Rao VK, Shankar SR, Taneja R Tags: Methods Mol Biol Source Type: research