Identification of a 5 ‑microRNA signature and hub miRNA‑mRNA interactions associated with pancreatic cancer.

Identification of a 5‑microRNA signature and hub miRNA‑mRNA interactions associated with pancreatic cancer. Oncol Rep. 2018 Oct 24;: Authors: Ma X, Tao R, Li L, Chen H, Liu Z, Bai J, Shuai X, Wu C, Tao K Abstract miRNA‑gene axes have been reported to serve an important role in the carcinogenesis of pancreatic cancer (PC). The aim of the present study was to systematically identity the microRNA signature and hub molecules, as well as hub miRNA‑gene axes, and to explore the potential biomarkers and mechanisms associated with the carcinogenesis of PC. Eleven microRNA profile datasets were obtained from the National Center for Biotechnology Information (NCBI) Gene Expression Omnibus (GEO) and ArrayExpress databases, and a meta‑analysis was performed to identify the differentially expressed miRNAs (DEMs) between tumor tissue and normal tissue. Subsequently, a diagnostic regression model was constructed to identify PC based on The Cancer Genome Atlas (TCGA) miRNA sequence data by using the least absolute shrinkage and selection operator (LASSO) method. In addition, GSE41368 was downloaded, and a weighted gene co‑expression network analysis (WGCNA) was performed to obtain the gene module associated with carcinogenesis by using the TCGAbiolinks and WGCNA packages, respectively. Finally, miRNA‑gene networks were constructed and visualized using Cytoscape software, followed by Gene Ontology (GO) and Kyoto Encyclopedia of Genes an...
Source: Oncology Reports - Category: Cancer & Oncology Tags: Oncol Rep Source Type: research