Generating Genetically Engineered Mice Using a Spermatogonial Stem Cell-Mediated Method.

Generating Genetically Engineered Mice Using a Spermatogonial Stem Cell-Mediated Method.
Methods Mol Biol. 2019;1874:87-98
Authors: Sato T, Ogawa T
Abstract
Mouse spermatogonial stem cells (SSCs) can be grown in culture for long periods. Cultured SSCs, also called germline stem (GS) cells, maintain themselves by self-renewing proliferation while retaining the ability to differentiate into sperm. Thus, when transplanted into the seminiferous tubules of a host mouse testis, they settle in the basal compartment of the tubules and establish spermatogenenic colonies. The sperm produced in the host are competent to produce offspring. This can be exploited for the generation of genetically modified mice, through the transplantation of genetically modified GS cells. In this section, we describe a method of genome editing-mediated GS cell modification and transplantation.
PMID: 30353509 [PubMed - in process]

Source: Mol Biol Cell - October 27, 2018 Category: Molecular Biology Authors: Sato T, Ogawa T Tags: Methods Mol Biol Source Type: research