Generation of Rabbit Models by Gene Editing Nucleases.
Generation of Rabbit Models by Gene Editing Nucleases.
Methods Mol Biol. 2019;1874:327-345
Authors: Yang D, Xu J, Chen YE
Abstract
Due to the lack of germline transmitting pluripotent stem cells (PSCs) cell lines and the extreme difficulty of somatic cell nuclear transfer (SCNT) in rabbit, the gene targeting technology in rabbit was lagging far behind those in rodents and in farm animals. As a result, the development and application of genetically engineered rabbit model are much limited. With the advent of gene editing nucleases, including ZFN, TALEN, and CRISPR/Cas9, it is now possible to produce gene targeting (i.e., knockout, knockin) rabbits with high success rates. In this chapter, we describe a comprehensive, step-by-step protocol for rabbit genome editing based on gene editing nucleases with specific emphasis of CRISPR/Cas9.
PMID: 30353523 [PubMed - in process]
Source: Mol Biol Cell - Category: Molecular Biology Authors: Yang D, Xu J, Chen YE Tags: Methods Mol Biol Source Type: research