Electrofusion of 2-Cell Embryos for Porcine Tetraploid Embryo Production.

Electrofusion of 2-Cell Embryos for Porcine Tetraploid Embryo Production.
Methods Mol Biol. 2019;1874:361-371
Authors: Kong Q, Liu Z
Abstract
The electrofusion of 2-cell embryos proves to be a simple and efficient way of generating mammalian tetraploid embryos. Many factors affect the fusion efficiency, such as fusion medium, electric field intensity, and fusion pulse length. In mice, production of tetraploid embryos by electrofusion has already been investigated; however, the investigation to produce porcine tetraploid embryos is seldom reported. In this chapter, we will describe oocytes in vitro maturation, in vitro fertilization, and the optimum conditions for electrofusion of 2-cell embryos to produce tetraploid embryos in pig.
PMID: 30353525 [PubMed - in process]

Source: Mol Biol Cell - October 27, 2018 Category: Molecular Biology Authors: Kong Q, Liu Z Tags: Methods Mol Biol Source Type: research