Identification of bacteria in juice/lettuce using magnetic nanoparticles and selected reaction monitoring mass spectrometry

This study presents an accurate means for bacterial identification in food matrixes using MS. The analysis time is less than 90 min and the minimum concentration of E. coli detected was 5 × 103 CFU/mL. The interaction between bacteria and the magnetic nanoparticles was electrostatic and nonspecific, in contrast to immunoassays which require specific antibodies. The targeted peptide analysis focuses on the bacterial markers, thus significantly simplifying the analysis and leading to an accurate identification of bacteria.Graphical abstract
Source: Journal of Food and Drug Analysis - Category: Food Science Source Type: research