Automatic cell nuclei segmentation and classification of cervical Pap smear images

Publication date: February 2019Source: Biomedical Signal Processing and Control, Volume 48Author(s): Pin Wang, Lirui Wang, Yongming Li, Qi Song, Shanshan Lv, Xianling HuAbstractPathological examination of microscopic image of Pap smear slide remains the main method for cervical cancer diagnosis. The accurate segmentation and classification of images are two important phases of the analysis. Firstly, the Mean-Shift clustering algorithm is applied to obtain regions of interest (ROI) for cell nuclei segmentation. Then the flexible mathematical morphology is applied to split overlapped cell nuclei for better accuracy and robustness. For classification of the images, features based on shape, textural features based on color space and Gabor features are extracted and put together to obtain better classification performance. The optimal feature set is obtained by chain-like agent genetic algorithm (CAGA), P-value and maximum relevance-minimum multicollinearity (MRmMC). The proposed segmentation and classification methods were tested on 362 cervical Pap smear images. Experimental results showed that the cervical cell nuclei can be segmented by the proposed segmentation method with high effective segmentation results (Sensitivity: 94.25%±1.03% and Specificity 93.45%±1.14%). The feature selection method based on CAGA with Gabor features has the highest classification performance for normal, uninvolved and abnormal images (more than 96% accuracy). The proposed method can automatically...
Source: Biomedical Signal Processing and Control - Category: Biomedical Science Source Type: research