Serological Diagnostics of Lyme Borreliosis: Comparison of Universal and Borrelia Species-Specific Tests Based on Whole-Cell and Recombinant Antigens Immunoassays

The study compares diagnostic parameters of different commercial serological kits based on three different antigen types and correlates test results with the status of the patient's Borrelia infection. In total, 8 IgM and 8 IgG kits were tested, as follows: enzyme-linked immunosorbent assay (ELISA) (Euroimmun) based on whole-cell antigen, 3 species-specific enzyme immunoassays (EIAs) (TestLine), Liaison chemiluminescence (DiaSorin), ELISA-Viditest (Vidia), EIA, and Blot-Line (TestLine) using recombinant antigens. All tests were performed on a panel of 90 samples from patients with clinically characterized borreliosis (53 with neuroborreliosis, 32 with erythema migrans, and 5 with arthritis) plus 70 controls from blood donors and syphilis patients. ELISA based on whole-cell antigens has superior sensitivity and superior negative predictive value and serves as an excellent screening test, although its specificity and positive predictive values are low. Species-specific tests have volatile parameters. Their low sensitivity and low negative predictive value handicap them in routine diagnostics. Tests with recombinant antigens are characterized by high specificity and high positive predictive value and have a wide range of use in diagnostic practice. Diagnostic parameters of individual tests depend on the composition of the sample panel. Only a small proportion of contradictory samples giving both negative and positive results is responsible for discrepancies between test results....
Source: Journal of Clinical Microbiology - Category: Microbiology Authors: Tags: Immunoassays Source Type: research

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Discussion The mandible normally grows in a symmetric downward and forward movement relative to the skull base. The condyle is the primary growth center. “The mandible is unique in that its 2 joints and growth centers function together as a single unit.” It is the last bone in the body to reach skeletal maturity. The mandible and its growth are important for maxillary growth and therefore many problems that affect the mandible affect the facial and skulls structures as well. These growth problems can be relatively insidious and therefore may need monitoring over longer periods of time such as patients with unde...
Source: PediatricEducation.org - Category: Pediatrics Authors: Tags: Uncategorized Source Type: news
AbstractContinued low-dose MMI treatment for longer than 12 –18 months may be considered in patients not in remission. However, ATDs are not free from adverse effects. We undertook a systematic review to clarify safety of long-term ATD treatment. Medline and the Cochrane Library for trials published between 1950 and Nov 2018 were systematically searched. We included original studies containing data for long-term (>  18 months) ATD treatment. Two reviewers independently extracted data from included trials and any disagreement was adjudicated by consensus. Of 615 related articles found, 12 fulfilled...
Source: Journal of Endocrinological Investigation - Category: Endocrinology Source Type: research
In this study, we found that cofilin competes with tau for direct microtubule binding in vitro, in cells, and in vivo, which inhibits tau-induced microtubule assembly. Genetic reduction of cofilin mitigates tauopathy and synaptic defects in Tau-P301S mice and movement deficits in tau transgenic C. elegans. The pathogenic effects of cofilin are selectively mediated by activated cofilin, as active but not inactive cofilin selectively interacts with tubulin, destabilizes microtubules, and promotes tauopathy. These results therefore indicate that activated cofilin plays an essential intermediary role in neurotoxic signaling th...
Source: Fight Aging! - Category: Research Authors: Tags: Newsletters Source Type: blogs
Called APPA, the pill is based on anti-inflammatory compounds found in peonies – flowers that brighten up flowerbeds in late spring and summer.
Source: the Mail online | Health - Category: Consumer Health News Source Type: news
This study not only provides prompts to the researcher who explores pharmacological and biological molecular mechanism of TWH applying to RA disease, but also proves a feasible method for discovering potential activated compounds from Chinese herbs. PMID: 31118961 [PubMed]
Source: Evidence-based Complementary and Alternative Medicine - Category: Complementary Medicine Tags: Evid Based Complement Alternat Med Source Type: research
LYME disease is a relatively unknown condition with an estimated 2,000 to 3,000 new cases each year in England and Wales. For an unknown disease however, anyone can develop the condition as its caught by a tick. This major symptom could mean you might have Lyme disease. Do you have it?
Source: Daily Express - Health - Category: Consumer Health News Source Type: news
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Source: AJN - Category: Nursing Tags: In the News Source Type: research
"Who knew there would be so much interest in a biomechanics paper??!!" Those are the words--and the multiple exclamation points--of Tara Moriarty, proprietor of the Moriarty Lab, an infectious diseases research lab that studies primarily Borrelia burgdorferi (Bb) the bacterium that causes Lyme disease. Moriarty is the principal investigator of a new academic paper from the University of Toronto that details the mechanism by which Bb "crawl" through the body. Scientific American is among the many mainstream outlets that discussed the findings, which may represent a major step in making t...
Source: Healthy Living - The Huffington Post - Category: Consumer Health News Source Type: news
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Source: Healthy Living - The Huffington Post - Category: Consumer Health News Source Type: news
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Source: Healthy Living - The Huffington Post - Category: Consumer Health News Source Type: news
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