Serological Diagnostics of Lyme Borreliosis: Comparison of Universal and Borrelia Species-Specific Tests Based on Whole-Cell and Recombinant Antigens Immunoassays

The study compares diagnostic parameters of different commercial serological kits based on three different antigen types and correlates test results with the status of the patient's Borrelia infection. In total, 8 IgM and 8 IgG kits were tested, as follows: enzyme-linked immunosorbent assay (ELISA) (Euroimmun) based on whole-cell antigen, 3 species-specific enzyme immunoassays (EIAs) (TestLine), Liaison chemiluminescence (DiaSorin), ELISA-Viditest (Vidia), EIA, and Blot-Line (TestLine) using recombinant antigens. All tests were performed on a panel of 90 samples from patients with clinically characterized borreliosis (53 with neuroborreliosis, 32 with erythema migrans, and 5 with arthritis) plus 70 controls from blood donors and syphilis patients. ELISA based on whole-cell antigens has superior sensitivity and superior negative predictive value and serves as an excellent screening test, although its specificity and positive predictive values are low. Species-specific tests have volatile parameters. Their low sensitivity and low negative predictive value handicap them in routine diagnostics. Tests with recombinant antigens are characterized by high specificity and high positive predictive value and have a wide range of use in diagnostic practice. Diagnostic parameters of individual tests depend on the composition of the sample panel. Only a small proportion of contradictory samples giving both negative and positive results is responsible for discrepancies between test results....
Source: Journal of Clinical Microbiology - Category: Microbiology Authors: Tags: Immunoassays Source Type: research

Related Links:

​​The company is marketing the drug as a replacement to Roche’s tocilizumab (originally approved for rheumatoid arthritis) that is currently in off-label use among Covid-19 patients who suffer from cytokine release syndrome (CRS).
Source: The Economic Times Healthcare and Biotech News - Category: Pharmaceuticals Source Type: news
Source: Pulmonary Medicine News - Doctors Lounge - Category: Respiratory Medicine Tags: Pulmonology, Rheumatology, News, Source Type: news
Survey finds many experiencing increased symptoms, less physical activity, emotional issues
Source: The Doctors Lounge - Psychiatry - Category: Psychiatry Tags: Family Medicine, Internal Medicine, Pharmacy, Psychiatry, Rheumatology, Journal, Source Type: news
MONDAY, July 13, 2020 -- Treatment with the immunosuppressant methotrexate does not raise the risk of lung disease among rheumatoid arthritis patients, two new studies claim. At least 5% to 10% of rheumatoid arthritis (RA) patients develop...
Source: - Daily MedNews - Category: General Medicine Source Type: news
A selective Janus kinase inhibitor, upadacitinib, is superior to methotrexate in improving clinical outcomes in patients with rheumatoid arthritis,  a study has suggested.
Source: The Pharmaceutical Journal - Category: Drugs & Pharmacology Source Type: research
Rheumatoid is a long-term joint condition that affects more than 400,000 people in the UK. It currently has no cure research shows that specific foods can alleviate symptoms.
Source: Daily Express - Health - Category: Consumer Health News Source Type: news
MONDAY, July 13, 2020 -- The Norfolk Arthritis Register has released an interim report on the impact of COVID-19 restrictions on people living with inflammatory arthritis who were advised to shelter at home due to increased vulnerability to COVID-19...
Source: - Pharma News - Category: Pharmaceuticals Source Type: news
"Who knew there would be so much interest in a biomechanics paper??!!" Those are the words--and the multiple exclamation points--of Tara Moriarty, proprietor of the Moriarty Lab, an infectious diseases research lab that studies primarily Borrelia burgdorferi (Bb) the bacterium that causes Lyme disease. Moriarty is the principal investigator of a new academic paper from the University of Toronto that details the mechanism by which Bb "crawl" through the body. Scientific American is among the many mainstream outlets that discussed the findings, which may represent a major step in making t...
Source: Healthy Living - The Huffington Post - Category: Consumer Health News Source Type: news
Late last month, FOX5NY broadcast "Lyme and Reason," a precedent-setting news special dedicated to the Lyme disease epidemic. The show was a success by many measures, including--most importantly by TV production standards--ratings wise. The strong viewership reflects a strong interest in learning more about Lyme disease. More and more, people are discovering that they know someone whose life has been profoundly affected by Lyme. More and more, people are discovering that they themselves have been living with undiagnosed Lyme for years. Now the FOX5NY team is calling on the U.S. Centers for Disease Control and pr...
Source: Healthy Living - The Huffington Post - Category: Consumer Health News Source Type: news
Today, the Boston Globe editorial board spoke out against a bill "that would require insurers in Massachusetts to cover Lyme disease treatments for as long as a doctor says they're needed." The Globe writes: Lawmakers may have good intentions, but they don't possess the expertise to decide whether it's beneficial, cost-efficient, or safe, to keep using strong medicines to treat a condition the medical establishment doubts is real. Such questions should be resolved by a consensus of doctors and researchers -- and buttressed by convincing clinical date [sic] -- not determined by legislative mandate. Who among the B...
Source: Healthy Living - The Huffington Post - Category: Consumer Health News Source Type: news
More News: Arthritis | Borrelia | Brain | Chemistry | Lyme Disease | Microbiology | Neurology | Rheumatology | Study | Syphilis