Modelling of ultraviolet light inactivation kinetics of methicillin ‐resistant Staphylococcus aureus, vancomycin‐resistant Enterococcus, Clostridium difficile spores and murine norovirus on fomite surfaces
Journal of Applied Microbiology, EarlyView.
Publication date: August 2020Source: Biomedicine &Pharmacotherapy, Volume 128Author(s): Mohammad Reza Farahpour, Ehsan Pirkhezr, Ali Ashrafian, Ali Sonboli
We present Prokaryotic Expression-profiling by Tagging RNA In Situ and sequencing (PETRI-seq), a high-throughput prokaryotic scRNA-seq pipeline. We demonstrated that PETRI-seq effectively barcoded single bacterial cells in a species-mixing experiment with E. coli (MG1655) and S. aureus (USA300). Within the S. aureus population, we found rare prophage induction in 0.04% of cells. We further demonstrated that PETRI-seq was able to distinguish between E. coli growth phases based on mRNA expression patterns by combining stationary E. coli with exponential E. coli in multiple experiments.
This study investigated the in vitro antimicrobial activity and heteroresistance risk of Eravacycline (Erava) in clinical E. faecalis isolates from China along with the mechanism of Erava resistance. A total of 276 non-duplicate E. faecalis isolates were retrospectively collected from a tertiary care hospital in China. Heteroresistance to Erava and the influence of tetracycline (Tet) resistance genes on Erava susceptibility were examined. To clarify the molecular basis for Erava resistance, E. faecalis variants exhibiting Erava-induced resistance were selected under Erava pressure. The relative transcript levels of six can...
CONCLUSIONS: The result of this work highlights the needed for new French recommendations based on the evolution of microbial epidemiology in the post PCV13 era. PMID: 32439250 [PubMed - as supplied by publisher]
Publication date: Available online 23 May 2020Source: The Journal of Molecular DiagnosticsAuthor(s): Giannoula S. Tansarli, Lindsay LeBlanc, Dianne B. Auld, Kimberle C. Chapin
Staphylococcus aureus is one of the main causative agents of mastitis in small ruminants. Antimicrobial use is the major treatment, but there are many flaws linked to resistance, tolerance or persistence. This st...
Publication date: Available online 22 May 2020Source: Sensors and Actuators B: ChemicalAuthor(s): Ling Chen, Yuan-Kui Leng, Bin Liu, Juan Liu, Sheng-Peng Wan, Tao Wu, Jinhui Yuan, Liyang Shao, Gu Guoqiang, Yong Qing Fu, Hengyi Xu, Yonghua Xiong, Xing-Dao He, Qiang Wu
Probiotics are living microorganisms which when taken in adequate amount provide various health benefits by maintaining the balance of bacteria in the intestine. Probiotics have countless health benefits some of which include improved digestion, enhanced immunity prevention of cancer and diabetes. The most common group of probiotics include species of Lactobacillus, Bifidobacterium and Enterococcus. In order to work as an effective probiotic, the microbial strain is expected to exhibit certain desirable characteristics like acid and bile tolerance, antimicrobial activity, adhesion to intestinal epithelium etc. Fermented pr...
Journal of Applied Microbiology,Volume 0, Issue ja, -Not available-.
We read with great interest the study by Ghamdi et al.  where the authors examined the bacterial contamination of computer keyboard and mouse of physician workstation at different intensive care units(ICUs). The contaminated surface environment in hospitals plays an important role in the transmission of methicillin-resistant Staphylococcus aureus(MRSA), vancomycin-resistant Enterococcus spp.(VRE), Clostridium difficile, Acinetobacter spp. and norovirus . Compute keyboard and mouse are high-touch surfaces in intensive care unit(ICU) and frequently contaminated by pathogens.