Role  of Annexin A2 isoform2 on the aggregative growth of dermal papillae cells.

Role of Annexin A2 isoform2 on the aggregative growth of dermal papillae cells. Biosci Rep. 2018 Oct 19;: Authors: Gu J, Ma Y, Yang L, Wang F, Lei C, Zhai J, Xia R Abstract Dermal papilla signals the follicular epithelial cells to prolong the hair growth process. Human Annexin A2 was preliminarily identified by 2-DE, MALDI-TOF-MS and database searching. The aim of the study was to explore the role of Annexin A2 in dermal papilla cells(DPC). RT-PCR and Western blot were adopted to detect the expression of Annexin A2. And siRNA technique was used to suppress the expression of Annexin A2. Over-expression vector was used to up-regulate the expression of Annexin A2. CCK 8 and PCNA were taken to detect the proliferation of DPC. The annexin A2 mRNA was up-regulated in passage 3 DPC. Western blot revealed that Annexin A2 isoform 2 was up-regulated in passage 3 DPC. The Annexin A2 isoform 2 siRNA was transfected into passage 3 DPC. RT-PCR data showed the mRNA expression of Annexin A2 isoform 2 was suppressed in DPC. Western blot showed the expression of Annexin A2 isoform 2 and PCNA were suppressed in DPC. CCK8 showed the proliferation of DPC was suppressed. Recombinant PLJM-Annexin A2 isoform 2 vector were transfected into passage 10 DPC. RT-PCR showed annexin A2 mRNA was up-regulated in DPC. Western blot showed the expression of annexin A2 isoform 2 and PCNA were up-regulated in DPC.CCK-8 showed the prolife...
Source: Bioscience Reports - Category: Biomedical Science Authors: Tags: Biosci Rep Source Type: research