Age-dependent changes in intervertebral disc cell mitochondria and bioenergetics.

Age-dependent changes in intervertebral disc cell mitochondria and bioenergetics. Eur Cell Mater. 2018 Oct 18;36:171-183 Authors: Hartman R, Patil P, Tisherman R, St Croix C, Niedernhofer LJ, Robbins PD, Ambrosio F, Van Houten B, Sowa G, Vo N Abstract Robust cellular bioenergetics is vital in the energy-demanding process of maintaining matrix homeostasis in the intervertebral disc. Age-related decline in disc cellular bioenergetics is hypothesised to contribute to the matrix homeostatic perturbation observed in intervertebral disc degeneration. The present study aimed to measure how ageing impacted disc cell mitochondria and bioenergetics. Age-related changes measured included matrix content and cellularity in disc tissue, as well as matrix synthesis, cell proliferation and senescence markers in cell cultures derived from annulus fibrosus (AF) and nucleus pulposus (NP) isolated from the discs of young (6-9 months) and older (36-50 months) New Zealand White rabbits. Cellular bioenergetic parameters were measured using a Seahorse XFe96 Analyzer, in addition to quantitating mitochondrial morphological changes and membrane potential. Ageing reduced mitochondrial number and membrane potential in both cell types. Also, it significantly reduced glycolytic capacity, mitochondrial reserve capacity, maximum aerobic capacity and non-glucose-dependent respiration in NP. Moreover, NP cells exhibited age-related decline in matrix synthesis and red...
Source: European Cells and Materials - Category: Cytology Tags: Eur Cell Mater Source Type: research