Quantifying the interactions between biomimetic biomaterials – collagen I, collagen IV, laminin 521 and cellulose nanofibrils – by colloidal probe microscopy

Publication date: 1 January 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 173Author(s): Robertus Wahyu N. Nugroho, Riina Harjumäki, Xue Zhang, Yan-Ru Lou, Marjo Yliperttula, Juan José Valle-Delgado, Monika ÖsterbergAbstractBiomaterials of different nature have been and are widely studied for various biomedical applications. In many cases, biomaterial assemblies are designed to mimic biological systems. Although biomaterials have been thoroughly characterized in many aspects, not much quantitative information on the molecular level interactions between different biomaterials is available. That information is very important, on the one hand, to understand the properties of biological systems and, on the other hand, to develop new composite biomaterials for special applications. This work presents a systematic, quantitative analysis of self- and cross-interactions between films of collagen I (Col I), collagen IV (Col IV), laminin (LN-521), and cellulose nanofibrils (CNF), that is, biomaterials of different nature and structure that either exist in biological systems (e.g., extracellular matrices) or have shown potential for 3D cell culture and tissue engineering. Direct surface forces and adhesion between biomaterials-coated spherical microparticles and flat substrates were measured in phosphate-buffered saline using an atomic force microscope and the colloidal probe technique. Different methods (Langmuir-Schaefer deposition, spin-coating, or adsorption) were app...
Source: Colloids and Surfaces B: Biointerfaces - Category: Biochemistry Source Type: research