Lectin-mediated in situ rolling circle amplification on exosomes for probing cancer-related glycan pattern.

Lectin-mediated in situ rolling circle amplification on exosomes for probing cancer-related glycan pattern. Anal Chim Acta. 2018 Dec 18;1039:108-115 Authors: Feng Y, Guo Y, Li Y, Tao J, Ding L, Wu J, Ju H Abstract Exosomal surface glycans play important roles in microvesicle protein sorting and exosome-cell interactions, and also provide promising biomarkers for various diseases. However, in situ detection techniques for exosomal glycans are largely lacking. In this work, an exosomal array is fabricated for probing cancer-related exosomal glycan signatures by lectin recognition-mediated in situ rolling circle assembly of fluorophore-labeled DNA. Different from commonly used lectin array, the proposed strategy enables the direct and amplified conversion of glycan recognition signals to fluorescence detection signals. Focusing on tumor-associated glycans including sialic acids, fucose and truncated O-glycans, the method has been used not only to compare glycan patterns between exosomes with different origins, but also to reveal the specific exosomal glycan characteristics compared to their parent cells. The limits of detection were identified to be 5.4 × 106 and 1.3 × 106 particles mL-1 for HeLa and PANC-1 exosomes, respectively. The dynamic ranges were 4.7 × 105 to 4.7 × 108, 4.7 × 108 to 4.7 × 109 for HeLa exosomes, and 4.7 × 105 to 1.2 × 109, 1.2 × 109 to 4.7 × 109 particles mL-1 for...
Source: Analytica Chimica Acta - Category: Chemistry Authors: Tags: Anal Chim Acta Source Type: research