Assessment of uveal melanomas using advanced diffusion-weighted imaging techniques: value of reduced field of view DWI ("zoomed DWI") and readout-segmented DWI (RESOLVE).

Assessment of uveal melanomas using advanced diffusion-weighted imaging techniques: value of reduced field of view DWI ("zoomed DWI") and readout-segmented DWI (RESOLVE). Acta Radiol. 2018 Oct 15;:284185118806666 Authors: Seeger A, Batra M, Süsskind D, Ernemann U, Hauser TK Abstract Background Diffusion-weighted imaging (DWI) has become an important tool for lesion characterization. Advanced techniques of DWI may be used to improve image quality. Purpose To evaluate multi-shot segmented DWI (rs-EPI, RESOLVE) and reduced field-of view DWI (rFOV-EPI, "zoomed" EPI) in patients with ocular melanoma and to compare image quality and diagnostic performance in differentiation of melanoma from retinal detachment. Material and Methods In this prospective and IRB-approved trial, we examined 26 patients using methods including conventional single-shot echo-planar DWI (ss-EPI), rs-EPI, and rFOV-EPI. Subjective image quality was compared using a four-point score and the maximum tumor length was measured in all sequences. Apparent diffusion coefficient (ADC) measurements were performed using a region-of interest analysis. Tumor delineation and differences in ADC values between melanomas and retinal detachments were compared. Results Diffusion restriction was markedly reduced in melanomas in all applied image techniques. Subjective image quality was significantly higher for rFOV-EPI (score = 3.5 ± 0.5) compared...
Source: Acta Radiologica - Category: Radiology Authors: Tags: Acta Radiol Source Type: research

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Authors: Kowal J, Markiewicz A, Dębicka-Kumela M, Bogdali A, Jakubowska B, Karska-Basta I, Romanowska-Dixon B Abstract Purpose: To perform a retrospective analysis of factors which might affect the occurrence of a relapse of uveal melanoma after 125I brachytherapy. Material and methods: The analysis concerned 343 patients treated in the years 2001-2012. The effect on local recurrence of such factors as patient's sex, age, tumour size, shape, pigmentation, location, presence of orange pigment or petechiae on tumour surface, retinal detachment, and blood or dispersed pigment in vitreous body were studied. Additi...
Source: Journal of Contemporary Brachytherapy - Category: Cancer & Oncology Tags: J Contemp Brachytherapy Source Type: research
Publication date: Available online 22 January 2020Source: The Journal of Molecular DiagnosticsAuthor(s): Aaron B. Beasley, Jacqueline Bentel, Richard J.N. Allcock, Tersia Vermeulen, Leslie Calapre, Timothy Isaacs, Melanie R. Ziman, Fred K. Chen, Elin S. GrayAbstractAnalysis of specific somatic copy number alterations (SCNAs) using multiplex ligation-dependent probe amplification (MLPA) is routinely used as prognostic test for uveal melanoma (UM). This technique requires relatively large amounts of input DNA, unattainable from many small fine needle aspirate biopsies. Herein we compared the use of MLPA with whole genome amp...
Source: The Journal of Molecular Diagnostics - Category: Pathology Source Type: research
LUCAT1 modulates miR ‐514a/b‐3p expression by serving as a sponge in CM. RBX1 is the downstream target gene of miR‐514a/b‐3p in CM. AbstractLong noncoding RNAs (lncRNAs) are essential regulators of gene expression and biological behaviors. However, the contribution of lncRNA LUCAT1 to choroidal melanoma (CM) remains unexplored. Here, we examined the expression of LUCAT1 in CM cells by qRT ‐PCR and investigated its biological effects by cell counting kit‐8, EdU, TUNEL, transwell assays, and Western blot. Bioinformatics tools were applied to find RNA candidates for further study. Moreover, mechanistic experiments...
Source: Cancer Medicine - Category: Cancer & Oncology Authors: Tags: ORIGINAL RESEARCH Source Type: research
Purpose: To study the relationship between gene expression profile (GEP) subclass and American Joint Committee on Cancer (AJCC) stage in patients with uveal melanoma (UM). Methods: A retrospective, multicenter study was undertaken with patients entered from nine major ocular oncology centers from across the United States. Three hundred sixty eligible patients had UM and underwent I-125 plaque brachytherapy with concurrent tumor biopsy with GEP testing between January 1, 2010, and October 28, 2014. Patient demographics and UM features were analyzed by both GEP and AJCC status. Results: Gene expression profile class ...
Source: RETINA - Category: Opthalmology Tags: Original Study Source Type: research
Analysis of specific somatic copy number alterations (SCNAs) using multiplex ligation-dependent probe amplification (MLPA) is routinely used as prognostic test for uveal melanoma (UM). This technique requires relatively large amounts of input DNA, unattainable from many small fine needle aspirate biopsies. Herein we compared the use of MLPA with whole genome amplification (WGA) combined with low-pass whole genome sequencing (LP-WGS) for detection of SCNA profiles in UM biopsy specimens. DNA was extracted from 21 formalin-fixed paraffin-embedded FFPE UM samples and SCNAs were assessed using MLPA and WGA followed by LP-WGS.
Source: Journal of Molecular Diagnostics - Category: Pathology Authors: Tags: Regular Article Source Type: research
In this study, we used bioinformatic methods to investigate the immune infiltration of uveal melanoma samples in public datasets. We first performed Gene Set Enrichment/Variation Analyses to detect immunological pathways that are altered in tumors with monosomy 3 or BAP1 deficiency. We then conducted an unsupervised clustering analysis to identify distinct immunologic molecular subtypes of uveal melanoma. We used CIBERSORT and ESTIMATE with RNA-seq data from The Cancer Genome Atlas and the GSE22138 microarray dataset to determine the sample-level immune subpopulations and immune scores of uveal melanoma samples. The Kaplan...
Source: Aging - Category: Biomedical Science Authors: Tags: Aging (Albany NY) Source Type: research
The authors have made their dataset available through Zenodo, an open-access repository developed under the European OpenAIRE program: “Dataset for ‘Validation of a Prognostic Staging for Metastatic Uveal Melanoma’: A Collaborative Study of the European Ophthalmic Oncology Group” (https://doi.org/10.5281/zenodo.3533542).
Source: American Journal of Ophthalmology - Category: Opthalmology Authors: Source Type: research
ConclusionAlthough this trial met the primary endpoint, the RR was still unsatisfactory. Therefore, further treatment development is required.
Source: International Journal of Clinical Oncology - Category: Cancer & Oncology Source Type: research
This case report describes a melanotic choroidal metastasis in the right eye.
Source: JAMA Ophthalmology - Category: Opthalmology Source Type: research
CONCLUSION: Select homoisoflavonoids have promise as antiangiogenic agents that are not generally cytotoxic. PMID: 31927011 [PubMed - as supplied by publisher]
Source: Fitoterapia - Category: Biochemistry Authors: Tags: Fitoterapia Source Type: research
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