Genotyping of Riemerella anatipestifer by ERIC-PCR and correlation with serotypes.

Genotyping of Riemerella anatipestifer by ERIC-PCR and correlation with serotypes. Avian Pathol. 2018 Oct 17;:1-9 Authors: Magyar T, Gyuris É, Ujvári B, Metzner M, Wehmann E Abstract Riemerella anatipestifer (RA) is a widely distributed bacterial pathogen of birds responsible for remarkable losses to poultry production, especially among waterfowl. We characterized the genomic diversity of 166 field isolates of RA collected from geese and ducks using enterobacterial repetitive intergenic consensus (ERIC)-polymerase chain reaction (PCR). The field strains and five reference strains showed 17 distinct patterns consisting of five to 12 bands ranging from approximately150-1800bp. The majority of the strains belonged to two closely related ERIC-PCR types (A and B), while the other types contained only a few isolates each. There was no association between ERIC-PCR type and host species, place, or year of isolation; however the ERIC-PCR pattern was correlated with serotype for most isolates. The majority of serotype 1 strains (101/107) belonged to ERIC-PCR type A while the remaining six strains represented five different ERIC-PCR types (D, G, L, M, and O). Serotypes 1,7 and 7 corresponded to ERIC-PCR types B and C, respectively. Serotypes 2, 4, and 10 could be subdivided by ERIC-PCR revealing two to four patterns within each serotype. These results indicate that ERIC-PCR may be a suitable technique for the molecular identification of RA se...
Source: Avian Pathology - Category: Pathology Authors: Tags: Avian Pathol Source Type: research