Cellular Magnetic Resonance Imaging for Tracking Metastatic Cancer Cells in the Brain.

Cellular Magnetic Resonance Imaging for Tracking Metastatic Cancer Cells in the Brain.
Methods Mol Biol. 2019;1869:239-251
Authors: Parkins KM, Makela AV, Hamilton AM, Foster PJ
Abstract
Cellular magnetic resonance imaging (MRI) enables visualization of cells in vivo. This is accomplished by labeling cells with superparamagnetic iron oxide nanoparticles. Here, we describe the steps for labeling human cancer cells with iron for tracking them after injection into nude mice. We also provide details for validation of cell labeling, ultrasound guided intra-cardiac injection, and MRI.
PMID: 30324528 [PubMed - in process]

Source: Mol Biol Cell - October 17, 2018 Category: Molecular Biology Authors: Parkins KM, Makela AV, Hamilton AM, Foster PJ Tags: Methods Mol Biol Source Type: research