Protective effect of royal jelly on the DNA integrity of sperms and early in vitro embryonic development in ofloxacin treated rats

This study was designed to determine the protective effects of royal jelly on the DNA integrity of sperms and early in vitro embryonic development in ofloxacin treated rats. In this study, 32 adult male rats were used and randomly allocated into 4 groups, each consisted of eight rats and dosed daily for 28  days by oral gavage: control—distilled water (216 mg/kg BW); ofloxacin group—ofloxacin (216 mg/kg BW); royal jelly group—royal jelly (100 mg/kg BW); royal jelly with ofloxacin group—216 mg/kg BW ofloxacin and 100 mg/kg BW royal jelly. The results showed that sperm viability, sperm pop ulation, sperm maturation, DNA maturity, hatching, IVF, GSH, and blastocyst significantly decreased (P <  0.05) in the ofloxacin group compared to the control. However, ofloxacin only caused significant increases (P <  0.05) in testis weight, immature sperm, and DNA damage of sperm compared to control group. In the ofloxacin with royal jelly group, there were not any important alterations detected and all sperm parameter returned to normal range. Royal jelly has protective effects on the DNA maturity of sperms and early in vitro embryonic development in ofloxacin treated rats.

Source: Comparative Clinical Pathology - October 17, 2018 Category: Pathology Source Type: research