5-lipoxygenase-dependent biosynthesis of novel 20:4 n-3 metabolites with anti-inflammatory activity
In this study, control and 5-LO-expressing HEK293 cells were stimulated in the presence of 20:4 n-3. Metabolites were characterized by LC-MS/MS and their anti-inflammatory properties assessed using AA-induced autocrine neutrophil stimulation and leukotriene B4-mediated chemotaxis. 8-hydroxy-9,11,14,17-eicosatetraenoic acid (Δ17-8-HETE) and 8,15-dihydroxy-9,11,13,17-eicosatetraenoic acid (Δ17-8,15-diHETE) were identified as novel metabolites. Δ17-8,15-diHETE production was inhibited by the leukotriene A4 hydrolase inhibitor SC 57461A. Autocrine neutrophil leukotriene stimulation and neutrophil chemotaxis, both BLT1-dependent processes, were inhibited by Δ17-8,15-diHETE at low nM concentrations. These data support an anti-inflammatory role for Δ17-8,15-diHETE, a novel 5-LO product.
Shaney L. Barratt, Sarah Mulholland, Khaled Al Jbour, Henry Steer, Markus Gutsche, Noeleen Foley, Rajiv Srivastava, Charles Sharp, Huzaifa I. Adamali
Pei-Li Zhu, Xiu-Qiong Fu, Jun-Kui Li, Anfernee Kai-Wing Tse, Hui Guo, Cheng-Le Yin, Ji-Yao Chou, Ya-Ping Wang, Yu-Xi Liu, Ying-Jie Chen, Muhammad Jahangir Hossen, Yi Zhang, Si-Yuan Pan, Zong-Jie Zhao, Zhi-Ling Yu
Yiming Chen, Yuping Jia, Weiguo Song, Lei Zhang
Jhana O. Hendrickx, Jaana van Gastel, Hanne Leysen, Paula Santos-Otte, Richard T. Premont, Bronwen Martin, Stuart Maudsley
Publication date: Available online 17 December 2018Source: Asian Journal of Pharmaceutical SciencesAuthor(s): Lipeng Qiu, Lu Ge, Miaomiao Long, Jing Mao, Kamel S. Ahmed, Xiaotian Shan, Huijie Zhang, Li Qin, Guozhong Lv, Jinghua ChenAbstractHeparosan is a natural precursor of heparin biosynthesis in mammals. It is stable in blood circulation but can be degraded in lysosomes, showing good biocompatibility and long circulation features. So heparosan can be designed as anticancer drug carriers to increase tumor selectivity and improve the therapeutic effect. A novel redox-sensitive heparosan-cystamine-vitamin E succinate (KSV)...
Conclusion: Our findings are the first report of co-segregation of the mutation in 6 family members, supporting its pathogenic role.Cardiology 2018;141:150 –155
Kelvin M. Jones, Balasubramanyam Karanam, Jacqueline Jones-Triche, Maninder Sandey, Henry J. Henderson, Rajeev S. Samant, Samuel Temesgen, Clayton Yates, Deepa Bedi
Marie-France Penet, Balaji Krishnamachary, Flonn é B. Wildes, Yelena Mironchik, Chien-Fu Hung, TC Wu, Zaver M. Bhujwalla
Lin Tuo, Jin Xiang, Xuanming Pan, Qingzhu Gao, Guiji Zhang, Yi Yang, Li Liang, Jie Xia, Kai Wang, Ni Tang