Molecules, Vol. 23, Pages 2650: Determination of 2 ′-Fucosyllactose and Lacto-N-neotetraose in Infant Formula

Molecules, Vol. 23, Pages 2650: Determination of 2′-Fucosyllactose and Lacto-N-neotetraose in Infant Formula
Molecules doi: 10.3390/molecules23102650
Authors:
Sean Austin
Denis Cuany
Julien Michaud
Bernd Diehl
Begoña Casado

Human milk oligosaccharides (HMO) are the third most abundant solid component of human milk. It is likely that they are responsible for at least some of the benefits experienced by breast-fed infants. Until recently HMO were absent from infant formula, but 2′-fucosyllactose (2′-FL) and lacto-N-neoteraose (LNnT) have recently become available as ingredients. The development of formula containing these HMO and the quality control of such formula require suitable methods for the accurate determination of the HMO. We developed two different approaches for analysis of 2′-FL and LNnT in formula; high performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) and hydrophilic interaction liquid chromatography with fluorescence detection (HILIC-FLD). In lab trials using blank formula spiked with the two oligosaccharides, both approaches worked well with recoveries of 94–111% (HPAEC-PAD) and 94–104% (HILIC-FLD) and RSD (iR) of 2.1–7.9% (HPAEC-PAD) and 2.0–7.4% (HILIC-FLD). However, when applied to products produced in a pilot plant, the HPAEC-PAD approach sometimes delivered results below those expected from the addition rate of the ingredients. We hypothesize that the oligosaccharides interact with the ...

Source: Molecules - October 16, 2018 Category: Chemistry Authors: Sean Austin Denis Cuany Julien Michaud Bernd Diehl Bego ña Casado Tags: Article Source Type: research