Purification of Cyclospora cayetanensis oocysts obtained from human stool specimens for whole genome sequencing

ConclusionsDensity gradients performed in the presence of a detergent followed by flow cytometry sorting of oocysts yielded sufficient genomic DNA largely free from contamination and suitable for whole genome sequencing ofC. cayetanensis. The methods described here will facilitate the accumulation of genomic sequences from various samples, which is a prerequisite for the development of typing tools to aid in outbreak investigations.
Source: Gut Pathogens - Category: Microbiology Source Type: research