Purification of Cyclospora cayetanensis oocysts obtained from human stool specimens for whole genome sequencing

ConclusionsDensity gradients performed in the presence of a detergent followed by flow cytometry sorting of oocysts yielded sufficient genomic DNA largely free from contamination and suitable for whole genome sequencing ofC. cayetanensis. The methods described here will facilitate the accumulation of genomic sequences from various samples, which is a prerequisite for the development of typing tools to aid in outbreak investigations.
Source: Gut Pathogens - Category: Microbiology Source Type: research

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In 2015 –2016, Zika virus (ZIKV) caused serious epidemics in Brazil. The key epidemiological parameters and spatial heterogeneity of ZIKV epidemics in different states in Brazil remain unclear. Early prediction...
Source: Parasites and Vectors - Category: Microbiology Authors: Tags: Research Source Type: research
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(Michigan Medicine - University of Michigan) Cryptosporidiosis, a diarrheal disease, is caused by the microscopic parasite cryptosporidium and it's a tough bug to killl. Two University of Michigan experts weigh in on how to prevent it's spreading.
Source: EurekAlert! - Medicine and Health - Category: International Medicine & Public Health Source Type: news
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Source: FT.com - Drugs and Healthcare - Category: Pharmaceuticals Source Type: news
The Centers for Disease Control and Prevention issued a warning about a rise in the number of outbreaks caused by the fecal parasite Cryptosporidium, or “Crypto.”
Source: Washington Post: To Your Health - Category: Consumer Health News Authors: Source Type: news
A recent announcement from the U.S. Centers for Disease Control and Prevention (CDC) may put a damper on summer fun. A fecal parasite often spread by swimming is causing an increasing number of illness outbreaks, the agency says. The number of disease outbreaks involving the parasite Cryptosporidium, also known as Crypto, increased by about 13% each year from 2009 to 2017, according to a new report from the CDC. People can become ill with cryptosporidiosis after exposure to contaminated human or animal fecal matter, developing symptoms including nausea, cramps and diarrhea that can last weeks and lead to serious malnutriti...
Source: TIME: Health - Category: Consumer Health News Authors: Tags: Uncategorized public health Source Type: news
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Source: Journal of Preventive Medicine and Hygiene - Category: International Medicine & Public Health Source Type: research
Abstract Cryptosporidium is a parasite that causes cryptosporidiosis, a profuse, watery diarrhea that can last up to 3 weeks in immunocompetent patients and can lead to life-threatening malnutrition and wasting in immunocompromised patients.* Fecal-oral transmission can occur by ingestion of contaminated recreational water, drinking water, or food, or through contact with infected persons or animals. For the period 2009-2017, public health officials from 40 states and Puerto Rico voluntarily reported 444 cryptosporidiosis outbreaks resulting in 7,465 cases. Exposure to treated recreational water (e.g., in pools an...
Source: MMWR Morb Mortal Wkl... - Category: Epidemiology Authors: Tags: MMWR Morb Mortal Wkly Rep Source Type: research
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