A validated LC-MS/MS method for simultaneous determination of linagliptin and metformin in spiked human plasma coupled with solid phase extraction: Application to a pharmacokinetic study in healthy volunteers

This study is concerned with the development of an HPLC-MS/MS method for simultaneous quantification of linagliptin and metformin in spiked human plasma. The method was applied to evaluate the potential pharmacokinetic interactions between the cited drugs in healthy volunteers. Solid phase extraction was applied using Strata™ X cartridge. Separation was carried out on Symmetry® C18 column using methanol: 10 mM ammonium formate buffer (containing 0.2% formic acid) in a ratio of (95: 5, v/v) as mobile phase at flow rate 0.25 mL min−1. Quantification was performed with multiple reaction monitoring in positive ionization mode. The monitored transitions were set at m/z 473.24 → 419.94, 130.14 → 60.18 and 340.27 → 116.07 for linagliptin, metformin and alogliptin (internal standard), respectively. The method was validated according to FDA guidelines. The method showed excellent linearity over concentration ranges 0.25–10 and 25–2000 ng mL−1 for linagliptin and metformin, respectively. The validated HPLC-MS/MS method was successfully applied to pharmacokinetic study of linagliptin and metformin in healthy volunteers after oral administration of Jentadueto® tablets.Graphical abstract
Source: Journal of Pharmaceutical and Biomedical Analysis - Category: Drugs & Pharmacology Source Type: research

Related Links:

CONCLUSIONS: Common comorbidities modify the association of frailty with postoperative survival; however, this effect was only apparent when analyses accounted for effect modification on the absolute risk difference, as opposed to relative scale. While the relative scale is more commonly used in biomedical research, smaller effects may be easier to detect on the risk difference scale. The concurrent presence of dementia, COPD, and HF with frailty were all associated with excess mortality on the absolute risk difference scale. PMID: 31743191 [PubMed - in process]
Source: Anesthesia and Analgesia - Category: Anesthesiology Authors: Tags: Anesth Analg Source Type: research
Publication date: Available online 19 November 2019Source: Chemical Engineering Research and DesignAuthor(s): Katarzyna Skrzypek, Efrem Curcio, Dimitrios StamatialisAbstractWidespread application of islet transplantation as a cure for type 1 Diabetes Mellitus patients is limited mainly due to the need for life-long immunosuppression associated with serious side effects. Immunoisolation by encapsulation of islets in semi-permeable membranes may overcome this issue as it allows for transplantation potentially obviating the need for immunosuppressive drug therapy.This work focuses on the development and validation of a model ...
Source: Chemical Engineering Research and Design - Category: Chemistry Source Type: research
As many as 10% to 20% of patients undergoing kidney transplantation develop post-transplant diabetes mellitus, which is associated with increased mortality. Even borderline changes in glucose metabolism, so-called prediabetes, may involve a similar risk. A recent study by Porrini et  al. showed for the first time that such changes in glucose metabolism are in fact associated with future cardiovascular disease and death in kidney-transplanted patients. This commentary discusses the relevance and clinical implications of these new findings.
Source: Kidney International - Category: Urology & Nephrology Authors: Tags: Commentary Source Type: research
Publication date: Available online 19 November 2019Source: Journal of Herbal MedicineAuthor(s): Bouzabata Amel, F. MahomoodallyAbstractThe aim of the study was to quantitatively document the use of plant-based medicine for the management of diabetes in Northeastern Algeria. Samples of 300 diabetic patients and 20 traditional healers were interviewed in five districts within Northeastern Algeria. The study showed that 4% of patients used phytotherapy in the management of type 2 diabetes mellitus (DM2), 26.3% recommended the amalgamation of conventional medicine with phytotherapy as a complementary or alternative medicine ag...
Source: Journal of Herbal Medicine - Category: Complementary Medicine Source Type: research
This study aimed to confirm the effectiveness and safety of metformin as a treatment option in patients with COPD. We performed a systematic search of PubMed, EMBASE, and the Cochrane database from their starting date to December 2017. Randomised controlled trials (RCTs), controlled clinical trials, and retrospective researches reporting the use of metformin for treating patients with COPD were identified. We included a total of six articles (involving 3,467 participants) and found that metformin may benefit patients with COPD and T2DM by improving health status and symptoms, hospitalisations, and mortality. There was no e...
Source: Journal of Thoracic Disease - Category: Respiratory Medicine Tags: J Thorac Dis Source Type: research
Publication date: Available online 20 November 2019Source: Clinica Chimica ActaAuthor(s): Qi Ma, Li Wang, Ye Yang, Yinxia Su, Tingting Wang, Qinqin Hou, Ren Cai, Zhiqiang Wang, Hua YaoAbstractObjectiveTo screen long non-coding RNA (lncRNA) related to glucokinase regulatory protein gene (GCKR), its differential expression was analyzed in patients with Type 2 diabetes mellitus (T2DM) and control samples. The correlation of lncRNA with GCKR was verified and its potential value as a molecular marker of T2DM was assessed.MethodsLymphocyte RNA was extracted from five patients with T2DM and five patients with non-T2DM. The expres...
Source: Clinica Chimica Acta - Category: Laboratory Medicine Source Type: research
Authors: Kaneko K, Chikamoto A, Hsu JC, Tochinai R, Sekizawa SI, Yamamoto M, Kuwahara M Abstract Environmental enrichment (EE) can reduce anxiety and stress in experimental animals, while little is known about the influence on autonomic nervous activity especially in disease animal models. Diabetes mellitus (DM) is associated with cardiovascular autonomic dysfunction, which can be characterized by a higher resting heart rate and a lower heart rate variability (HRV). We hypothesized that EE can enhance parasympathetic nervous activity while reducing disease progression in type 2 diabetic mice. A telemetry transmitte...
Source: Experimental Animals - Category: Research Tags: Exp Anim Source Type: research
This study is the first to examine the contribution of the HLA-DR/DQ alleles and haplotypes to T1D susceptibility in Jordanians. METHODS: Polymerase chain reaction sequence-specific primers (PCR-SSP) were used to genotype 41 Jordanian healthy controls and 50 insulin-dependent diabetes mellitus (IDDM) patients. RESULTS: The following alleles were found to be significant high risk alleles in T1D Jordanian patients: DRB1*04 (OR=3.95, p
Source: Endocrine, Metabolic and Immune Disorders Drug Targets - Category: Drugs & Pharmacology Tags: Endocr Metab Immune Disord Drug Targets Source Type: research
Contributors : Chen L Zheng ; Bi C ChenSeries Type : Expression profiling by arrayOrganism : Rattus norvegicusPost-transplantation diabetes mellitus (PTDM) is a known complication of transplantation which affected the prognosis. Tacrolimus (Tac, or FK506) is a widely used immunosuppressant, has been reported as a risk factor for PTDM and further develops complications in heart and skeletal muscle , while the mechanism is still largely unknown. The gene expression of rat muscles of control group and Tac induced PTDM group were analyzed.
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by array Rattus norvegicus Source Type: research
Authors: Doulberis M, Papaefthymiou A, Polyzos SA, Katsinelos P, Grigoriadis N, Srivastava DS, Kountouras J Abstract Obese or overweight people exceed one-third of the global population and obesity along with diabetes mellitus consist basic components of metabolic syndrome, both of which are known cardio-cerebrovascular risk factors with detrimental consequences. These data signify the pandemic character of obesity and the necessity for effective treatments. Substantial advances have been accomplished in preclinical research of obesity by using animal models, which mimic the human disease. In particular, rodent mod...
Source: Minerva Endocrinologica - Category: Endocrinology Tags: Minerva Endocrinol Source Type: research
More News: Diabetes | Diabetes Mellitus | Drugs & Pharmacology | Endocrinology | Fortamet | Metformin | Study