Development and application of a real-time polymerase chain reaction method for quantification of Escherichia coli in oysters (Crassostrea gigas).

The objective of this study was to develop a qPCR method for quantification of E. coli in oysters. Additionally, different methods were evaluated for DNA extraction from oyster samples and the more reliable method was chosen. Primers and probe were designed targeting uidA gene of E. coli and shown to specifically amplify DNA from E. coli. Standard curves with bacterial DNA extracted from oysters samples artificially inoculated with E. coli were conducted. A good correlation was noticed when the qPCR method was compared to a culture method in oyster samples. This is the first report of a method exclusively developed for direct quantification of E. coli in oyster, the method showed to be suitable for quantification of E. coli in oysters and could be useful in routine analyses, as it requires less time than the culture method.
PMID: 30297060 [PubMed - in process]

Source: Food Microbiology - October 10, 2018 Category: Food Science Authors: Miotto M, Fonseca AA, Barretta C, da Silva HS, Pellizzaro T, De Dea Lindner J, Vieira CRW, Parveen S, Prudencio ES Tags: Food Microbiol Source Type: research