Enhanced Delivery of Protein Fused to Cell Penetrating Peptides to Mammalian Cells.

Enhanced Delivery of Protein Fused to Cell Penetrating Peptides to Mammalian Cells. BMB Rep. 2018 Oct 08;: Authors: Moon JI, Han MJ, Yu SH, Lee EH, Kim SM, Han K, Park CH, Kim CH Abstract Recent progress in cellular reprogramming technology and lineage-specific cell differentiation has provided great opportunities for translational research. Because virus-based gene delivery is not a practical reprogramming protocol, protein-based reprogramming has been receiving attention as a safe way to generate reprogrammed cells. However, the poor efficiency of the cellular uptake of reprogramming proteins is still a major obstacle. Here, we reported key factors which improve the cellular uptake of these proteins. Purified red fluorescent proteins fused with 9xLysine (dsRED-9K) as a cell penetrating peptide were efficiently delivered into the diverse primary cells. Protein delivery was improved by the addition of amodiaquine. Furthermore, purified dsRED-9K was able to penetrate all cell lineages derived from mouse embryonic stem cells efficiently. Our data may provide important insights into the design of protein-based reprogramming or differentiation protocols. PMID: 30293549 [PubMed - as supplied by publisher]
Source: BMB Reports - Category: Biochemistry Authors: Tags: BMB Rep Source Type: research