Inflammatory production of reactive oxygen species by Drosophila hemocytes activates cellular immune defenses.

Inflammatory production of reactive oxygen species by Drosophila hemocytes activates cellular immune defenses.
Biochem Biophys Res Commun. 2018 Oct 03;:
Authors: Myers AL, Harris CM, Choe KM, Brennan CA
Abstract
The production of reactive oxygen species (ROS) is a prominent response to infection among innate immune cells such as macrophages and neutrophils. To better understand the relationship between antimicrobial and regulatory functions of blood cell ROS, we have characterized the ROS response to infection in Drosophila hemocytes. Using fluorescent probes, we find a biphasic hemocyte ROS response to bacterial infection. In the first hour, virtually all hemocytes generate a transient ROS signal, with nonphagocytic cells including prohemocytes and crystal cells displaying exceptionally strong responses. A distinct, and more delayed ROS response starting at 90 min is primarily within cells that have engulfed bacteria, and is sustained for several hours. The early response has a clear regulatory function, as dampening or intensifying the intracellular ROS level has profound effects on plasmatocyte activation. In addition, ROS are necessary and sufficient to activate JNK signalling in crystal cells, and to promote JNK-dependent crystal cell rupture. These findings indicate that Drosophila will be a promising model in which to dissect the mechanisms of ROS stimulation of immune activation.
PMID: 30292413 [PubMed - as supplied...

Source: Biochemical and Biophysical Research communications - October 3, 2018 Category: Biochemistry Authors: Myers AL, Harris CM, Choe KM, Brennan CA Tags: Biochem Biophys Res Commun Source Type: research