Low-power laser alters mRNA levels from DNA repair genes in acute lung injury induced by sepsis in Wistar rats

AbstractAcute lung injury (ALI) is defined as respiratory failure syndrome, in which the pathogenesis could occur from sepsis making it a life-threatening disease by uncontrolled hyperinflammatory responses. A possible treatment for ALI is the use of low-power infrared lasers (LPIL), whose therapeutical effects depend on wavelength, power, fluence, and emission mode. The evaluation mRNA levels of repair gene related to oxidative damage after exposure to LPIL could provide important information about the modulation of genes as treatment for ALI. Thus, the aim of this study was to evaluate the mRNA levels fromOGG1,APEX1,ERCC2, andERCC1 genes in lung tissue from Wistar rats affected by ALI and after exposure to LPIL (808  nm; 100 mW). Adult male Wistar rats (n = 30) were randomized into six groups (n = 5, for each group): control, 10 J/cm2 (2  J), 20 J/cm2 (5  J), ALI, ALI + LPIL 10 J/cm2 and ALI + LPIL 20  J/cm2. ALI was induced by intraperitonealE. coli lipopolysaccharide injection (10  mg/kg). Lungs were removed, and samples were withdrawn for total RNA extraction, cDNA synthesis, and mRNA levels were evaluated by RT-qPCR. Data normality was verified by Kolmogorov-Smirnov, comparisons among groups were by Student’st test, Mann-Whitney test, one-way ANOVA, Kruskal-Wallis followed by post-tests. Data showed that OGG1 (0.39  ± 0.10), ERCC2 (0.67 ± 0.24), and ERCC1 (0.60 ± 0.19) mRNA levels are reduced in ALI group when compared with the control ...
Source: Lasers in Medical Science - Category: Laser Surgery Source Type: research